FokI内切酶报价

特别提示：包括FokI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：FokI内切酶
英文名称：FokI
产品货号：GS2181
产品规格：200U

储存条件：冷冻（-20℃）

使用说明

Overdigestions of>5 units of Fok I per 1μgof DNA and incubation times>2hoursare not recommended.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	GGATG(N)9↑
来源	E.coli strain that carries the cloned Fok I gene from Flavobacterium okeanokoites
Assayed on	Lambda DNA
Optimal buffer	Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 2-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a.of enzyme for 16 hours at 37℃
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	BseGI^,BstF5I^,BtsCI^

我公司销售的FokI内切酶报价，FokI限制性内切酶质量上佳，价格普惠，欢迎广大新老客户踊跃订购。