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- 2026年03月10日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Porcine aortic smooth muscle cell line
- 库存：
10
- 供应商：
欣润生物
- 肿瘤类型：
NO
- 细胞类型：
永生化
- ATCC Number：
11222
- 品系：
巴马猪
- 组织来源：
主动脉
- 相关疾病：
无
- 物种来源：
猪
- 免疫类型：
不详
- 细胞形态：
梭形
- 是否是肿瘤细胞：
否
- 器官来源：
血管
- 运输方式：
常温
- 年限：
成年
- 生长状态：
贴壁生长
- 规格：
T25方瓶
永生化猪主动脉平滑肌细胞简介：
产品描述：平滑肌细胞对心血管疾病的发生起着极其重要的作用。心血管疾病的发生和发展的一个主要因素在于心血管平滑肌细胞转变成为了具有增殖能力的表型。近期的研究表明，平滑肌细胞能表达钙离子通道ICAM-1和VCAM-1，其中ICAM-1和VCAM-1的表达可能是造成血管壁炎症反应，并进一步造成心血管疾病的原因。因此，对血管平滑肌细胞的体外培养和研究可用来发现和确定新的血管疾病的靶向治疗方法。
产品货号：IP4009
产品类型: 永生化细胞
传代能力: 30代左右
产品形态: 梭形
培养基：永生化猪主动脉平滑肌细胞完全培养基
支原体质控：呈阴性
产品培养条件：37℃，5%CO2
发货方式：T25瓶子常温发货
货期：1周左右货期
永生化猪主动脉平滑肌细胞白光图
Variables controlling the secretion of a somatomedin-like peptide by cultured porcine smooth muscle cells
Somatomedin-C, a peptide growth factor that is also termed insulin-like growth factor I, stimulates smooth muscle cell replication; however, these cells proliferate in somatomedin-C-deficient medium. We postulated, therefore, that these cells might release a somatomedin-like molecule into the surrounding culture medium. Porcine aortic smooth muscle cells that were exposed to serum-free Dulbecco's modified minimum essential medium for 24 hours were found to release a somatomedin-like molecule that could be detected by a specific radioimmunoassay for somatomedin-C. Several variables in the design of tissue culture conditions were found to regulate the secretion of the somatomedin-like peptide. There was an inverse relationship between somatomedin-like peptide secretion and culture density. Cultures grown to a density of 110,000 cells/well secreted 0.18 +/- 0.02 U/ml per 10(5) cells, whereas cultures grown to 38,000 cells/well secreted 0.59 +/- 0.06 U/ml per 10(5) cells (P less than 0.001). Serum deprivation and days of preincubation before initiation of
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文献和实验Chicken intestinal epithelial cells were obtained from NEWGAINBIO company. Cells were cultured on 37℃, with 5% CO2, in the Ham’s F-12 Nutrient (DMEM/12) that contained the following supplementations: fetal bovine serum (5%), in-sulin (5 µg/mL), transferrin (5 µg/mL), selenium (5 ng/mL), epidermal growth factor (5 ng/mL) and penicillin-streptomycin (100–100 U/mL) for cell culturing (full DMEM/12). Experiments were performed with chicken intestinal epithelial cells and working solutions were prepared with plain DMEM/12 without supplementation. For the investigations, cells were seeded onto 96-well, 24-well or 6-well polystyrene cell culture plates.
Primary hVICs (passage 2) were cultured to 50–60% confluence and infected with pGMLV-SV40T-puro lentivirus (NewgainBio, Wuxi, China) at a multiplicity of infection of 80 supplemented with 5 µg/mL polybrene (Sigma-Aldrich, Buchs, Switzerland).
Tissue was cultured until cells became visible around the tissue, and when the fusion reached 90% (FIGURE 1A) §ask ¦lled with the prepared culturing medium was sent to the company for further immortalisation. Cell immortalisation was done for cell stability and longer-term use. Immortalised cells were cultured with 10% FBS and 1% PS in the DMEM medium. After the cells multiplied and merged, they were routinely passed and grown ( NEWGAINBIO Inc. Wuxi, Jiangsu, China) (FIGURE 1B-C).
Mouse primary cultured renal vascular ECs and VSMCs were obtained from Newgainbio company, which were tested by Factor VIII and α-smooth muscle actin (α-SMA), the marker of ECs and VSMCs. RNeasy Mini Kit was used for RNA extraction, and the above protocols were repeated.
Porcine primary colon epithelial cells (Newgainbio company, Wuxi,China) were cultured in Dulbecco's Modified Eagle's Medium (Solarbio, Beijing, China) containing 10 % fetal bovine serum (BioInd, Kiryat shmona, Lsrael) at 37 ◦C and 5 % CO2 humidity.
技术资料
