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肾脏
其他疾病
CRL-4004™
贴壁生长
angiomyolipoma
上皮样
36 years
人
0
冻存运输
大量
Designations: | UMB1949 | ||
Depositors: | JL Arbiser | ||
Biosafety Level: | 2 [cells contain SV40 viral DNA sequences ] | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | epithelial-like |
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Source: | Organ: kidney Tissue: angiomyolipoma Disease: tuberous sclerosis Immortalization method: introduction of telomerase into SV40-transfected angiomyolipoma cells |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
Isolation: | Isolation date: June 2004 | ||
Applications: | In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. As such, this cell line can be used to study signal transduction and drug efficiency in tuberous sclerosis. The UMB1949 cell line was established by sequential introduction of the SV40 large T antigen and human telomerase into human angiomyolipoma cells. |
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Antigen Expression: | positive for epithelial marker pan-cytokeratin (immunocytochemistry) | ||
DNA Profile (STR): | Amelogenin: XY CSF1PO: 10, 11 D13S317: 12, 13 D16S539: 12 D5S818: 11 D7S820: 9, 10 THO1: 6, 8 TPOX: 12 vWA: 18 |
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Cytogenetic Analysis: | This cell line is of male origin and 1/2 to 2/3 of the total cell population is pseudodiploid, the rest of the cells fall in the tetraploid range. Consistent cytogenetic changes include chromosome 10 and 19 aberration, and chromosome 4 monosomy. Some cells showed loss of the Y chromosome and many of the examined cells contained random chromosomal aberrations. | ||
Age: | 36 years | ||
Gender: | male | ||
Ethnicity: | Caucasian | ||
Comments: | The UMB1949 cell line was established by sequential introduction of the SV40 large T antigen and human telomerase into human angiomyolipoma cells. Angiomyolipomas are benign tumors of the kidney which originate from putative perivascular epithelioid cells that may undergo differentiation into cells with features of melanocytes, smooth muscle or fat cells. UMB1949 cells have a defined 5bp deletion in exon 33 of tuberin (Tsc2) and mutations in tuberin (and/or hamartin) cause tuberous sclerosis. As such, this cell line can be used to study signal transduction and drug efficiency in tuberous sclerosis. [16173554] As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. |
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Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:5 is recommended. Medium renewal: Every 2 to 3 days |
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Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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Doubling Time: | about 29 hours | ||
Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002 Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X Phosphate-buffered saline: ATCC 30-2200 |
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References: | 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 53507: Arbiser JL, et al. The generation and characterization of a cell line derived from a sporadic renal angiomyolipoma: use of telomerase to obtain stable populations of cells from benign neoplasms. Am. J. Pathol. 159: 483-491, 2001. PubMed: 11485907 16173554: Lim SD , et al. Expression of the neural stem cell markers NG2 and L1 in human angiomyolipoma: are angiomyolipomas neoplasms of stem cells? Mol. Med. 13(3-4): 160-165, 2007. PubMed: 17592550 |
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