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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 组织来源：
bronchus
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞形态：
上皮样
- 细胞类型：
其他细胞类型
- 器官来源：
肺
- 运输方式：
冻存运输
- 相关疾病：
囊肿性纤维化
- ATCC Number：
CRL-4016™
- 生长状态：
贴壁生长
- 年限：
32 years
| Designations: | CuFi-5 | ||
| Depositors: | AJ Klingelhutz | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial-like |
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| Source: | Organ: lung Tissue: bronchus Disease: cystic fibrosis Cell Type: epithelial immortalized with hTERT and HPV-16 E6/E7-LXSN |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to AT CC before shipment. The price listed above is for noncommercial and academic organizations only. Commerci al and for-profit organizations should call for pricing. | ||
| Isolation: | Isolation date: 2001 | ||
| Applications: | In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. Human airway epithelial (HAE) cell line, CuFi-5, was derived from lung of a 32-year-old patient with cystic fibrosis by retroviral infection with hTERT and HPV-16E6/E7. Consequently, the cells do not undergo growth arrest in cell culture due to exogenous expression of the telomerase and E6/E7 genes. Other hTERT-immortalized cell lines derived from cystic fibrosis HAE are also available from ATCC as ATCC CRL-4013 (CuFi-1), ATCC CRL-4015 (CuFi-4), and ATCC CRL-4017 (CuFi-6). |
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| Antigen Expression: | positive for epithelial marker pan-cytokeratin (immunocytochemistry) | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 10,14 D13S317: 11,13 D16S539: 11,13 D5S818: 11,12 D7S820:11,12 THO1: 7 TPOX: 8,10 vWA: 16,17 |
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| Cytogenetic Analysis: | This is a near-diploid cell line of male origin in which the most consistent karyotypic aberrations are trisomy of chromosomes 5 and 20. Other non-clonal aberrations were found at early passage, but the karyology tended to stabilize within several passages. | ||
| Age: | 32 years | ||
| Gender: | male | ||
| Comments: | Human airway epithelial (HAE) cell line, CuFi-5, was derived from lung of a 32-year-old patient with cystic fibrosis by retroviral infection with hTERT and HPV-16E6/E7. Consequently, the cells do not undergo growth arrest in cell culture due to exogenous expression of the telomerase and E6/E7 genes. [22566 ] CuFi-5 cells are homozygous for the delta F508 cystic fibrosis-causing mutation (delta F508/delta F508). Other hTERT-immortalized cell lines derived from cystic fibrosis HAE are also available from ATCC as ATCC CRL-4013 (CuFi-1), ATCC CRL-4015 (CuFi-4), and ATCC CRL-4017 (CuFi-6). As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. |
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| Propagation: | ATCC complete growth medium: These cells are grown in a serum-free medium: BEGM (Bronchial Epithelial Growth Medium, Serum-free) from Lonza (BEGM Bullet Kit; CC-3170) made of BEBM basal medium and SingleQuot additives (ATCC does not use gentamycin-amphotericin B) supplemented with 50 �g/ml G-418. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2 ), 5% |
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| Subculturing: | Protocol: Note: The culture flasks should be pre-coated with 60µg/ml solution of Human Placental Collagen Type IV. (Sigma, Cat. No. C-7521) at least 18 hours in advance then air-dried and rinsed 2-3 times with Dulbecco's Phosphate Buffered Saline.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended. Medium renewal: every 2 to 3 days |
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| Preservation: | Freeze medium: BEGM with 30% FBS and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | approximately 36 hours | ||
| Related Products: | Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X Phosphate-buffered saline: ATCC 30-2200 |
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| References: | 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 92666: Zabner J, et al. Development of cystic fibrosis and noncystic fibrosis airway cell lines . Am. J. Physiol. Lung Cell Mol Physiol. 284: L844-L854, 2003. PubMed: 12676769 92667: Kiyono T, et al. Both Rb/p161NK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 396: 84-88, 1998. PubMed: 9817205 |
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