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其他细胞类型
cartilage
大量
贴壁生长
成纤维样
正常
CRL-2846™
18 weeks fetus
骨
冻存运输
Designations: | CHON-001 | ||
Depositors: | ATCC | ||
Biosafety Level: | 2 [cells containing SV40 viral DNA sequences ] | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | fibroblast-like |
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Source: | Organ: bone Tissue: cartilage Disease: normal Cell Type: fibroblast immortalized with hTERT |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
Isolation: | Isolation date: December 20, 2001 | ||
Applications: | As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. The primary cells were infected by the defective retrovirus containing hTERT gene under G418 selection. The chondrocyte cell line, CHON-001, was derived from the long bones of an 18-week female fetus. Gene Expression: Positive for Collagen type I, Collagen type X, and aggrecan (RT-PCR). Negative for Collagen type II (RT-PCR). |
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Antigen Expression: | HLA-B58; Homo sapiens, expressed HLA-B81; Homo sapiens, expressed HLA-Cw6; Homo sapiens, expressed HLA-Cw8; Homo sapiens, expressed HLA-DR7; Homo sapiens, expressed HLA-DR12; Homo sapiens, expressed HLA-DR52; Homo sapiens, expressed HLA-DQ2; Homo sapiens, expressed HLA-DQ5; Homo sapiens, expressed |
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DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 12,14 D16S539: 11,13 D5S818: 11,12 D7S820: 12 THO1: 6,7 TPOX: 8,11 vWA: 16,17 |
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Cytogenetic Analysis: | This is a diploid cell line of female origin. Overall, the karyology is stable with a modal chromosome number of 46 in 93% of the examined cells and a low rate of polypoidy. No consistent structural chromosomal aberrations were found in any of the cells examined. | ||
Age: | 18 weeks fetus | ||
Gender: | female | ||
Comments: | The chondrocyte cell line, CHON-001, was derived from the long bones of an 18-week female fetus. The primary cells were infected by the defective retrovirus containing hTERT gene under G418 selection. The defective retrovirus was collected from the supernatant of the packaging cell line, PT67 transfected with the pLXSN vector containing hTERT gene. Gene Expression: Positive for Collagen type I, Collagen type X, and aggrecan (RT-PCR). Negative for Collagen type II (RT-PCR).As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. | ||
Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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Subculturing: | Protocol: Volumes are given for T75 sq.cm flasks; proportionally reduce or increase the amount of dissociation medium for culture vessels of other sizes.
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Preservation: | Freeze medium: 90% heat-inactivated fetal bovine serum; 10% DMSO Storage temperature: liquid nitrogen vapor phase |
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Doubling Time: | about 72 hours | ||
Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 Cell culture tested DMSO:ATCC 4-X Erythrosin B vital stain solution:ATCC 30-2404 Trypan Blue vital stain solution:ATCC 30-2402 |
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