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- 组织来源：
epithelium, pigmented
- ATCC Number：
CRL-4000™
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞形态：
上皮样
- 库存：
大量
- 细胞类型：
其他细胞类型
- 生长状态：
贴壁生长
- 器官来源：
其他
| Designations: | hTERT RPE-1 | ||
| Depositors: | Geron Corporation | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: retina, eye Tissue: epithelium, pigmented Cell Type: epithelial immortalized with hTERT |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
| Applications: | s part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141 ). |
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| Antigen Expression: | Ep-16 epithelial antigen; Homo sapiens, expressed (The Ep-16 antigen was assayed by flow cytometry using the Ep-16 monoclonal antibody (ATCC HB-155 )) (The Ep-16 antigen was assayed by flow cytometry using the Ep-16 monoclonal antibody (ATCC HB-155 )) cytokeratin; Homo sapiens, expressed (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody) (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody) |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 12,14 D13S317: 11,12 D16S539: 11 D5S818: 11 D7S820: 10,11 THO1: 9 TPOX: 8 vWA: 17,18 |
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| Cytogenetic Analysis: | This is a near-diploid human cell line of female origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes consist of a karyotypically normal X-chromosome and a derivative X-chromosome with additional chromosomal material at the terminal end of the q-arm. The derivative X-chromosome was present in all of the cells analyzed. | ||
| Gender: | female | ||
| Comments: | The hTERT-immortalized retinal pigment epithelial cell line, hTERT RPE-1, was derived by transfecting the RPE-340 cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141 ). Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332, 9916802]. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Subculture when cell concentration reaches between 2 X 10(4) and 4 X 10(4) cells/cm2. |
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:10 twice weekly Medium Renewal: every 2 days (or as needed) |
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| Preservation: | Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 19 hr | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 Trypsin-EDTA Solution:ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X Erythrosin B vital stain solution:ATCC 30-2404 Trypan Blue vital stain solution:ATCC 30-2402 plasmid in bacteria:ATCC MBA-141 Hanks' Balanced Salt Solution (HBSS): ATCC 30-2213 |
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| References: | 47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332 90421: Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802 90422: Matsunaga H, et al. Beta-galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells. Invest. Ophthalmol. Vis. Sci. 40: 197-202, 1999. PubMed: 9888444 |
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. However, the expression of the human telomerase reverse transcriptase (hTERT ) gene, which encodes the catalytic protein subunit of human telomerase, has been shown to be the major determining factor. To gain insight into the mechanisms regulating hTERT expression
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