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Barrett食管
CRL-4027™
人
0
大量
其他细胞类型
上皮样
食道
贴壁生长
epithelium
Designations: | CP-A (KR-42421) | ||
Depositors: | B Reid | ||
Biosafety Level: | 2 | ||
Shipped: | frozen | ||
Medium & Serum: | See Propagation | ||
Growth Properties: | adherent | ||
Organism: | Homo sapiens | ||
Morphology: | epithelial-like |
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Source: | Organ: esophagus Tissue: epithelium Cell Type: non-dysplastic metaplasia Disease: Barrett's esophagus Immortalization method: hTERT expression |
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Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
Restrictions: | This material requires that the Addendum for Commercial and For-Profit Organizations or the Addendum for Noncommercial and Academic Organizations be signed and returned to ATCC before shipment. The price listed above is for noncommercial and academic organizations only. Commercial and for-profit organizations should call for pricing. | ||
Isolation: | Isolation date: August 1995 | ||
Applications: | Genetic instability studies using flow cytometry and FISH reveal the retention of elevated tetraploidy (G2/tetraploidy) in the hTERT-immortalized cells, similar to the non-transduced parental cells. This cell line shows increased telomerase activity and long telomeres of about 12 kb as assessed by terminal restriction fragment lengths (TRF) analysis. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. The Barrett's esophagus cell line, CP-A (also identified as KR-42421 or QhTERT), was derived from an endoscopic biopsy specimen obtained from a region of non-dysplastic metaplasia and transduced with the retroviral expression vector, pLXSN-hTERT, to create an immortalized cell line. |
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Antigen Expression: | positive for epithelial marker pan-cytokeratin (immunocytochemistry)(verified at ATCC ) negative for gastric mucin (CHL2) (immunocytochemistry)(verified at ATCC ) |
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DNA Profile (STR): | Amelogenin: X,Y CSF1PO:12 D13S317:12,14 D16S539:12,13 D5S818:11,12 D7S820:7,8 THO1: 7,9.3 TPOX: 8,9 vWA: 17 |
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Cytogenetic Analysis: | This is a near-diploid cell line of male origin in which 2 sub-clones make up the majority of the cell population. One clone containing i(8)(q10) and trisomy 20 and the other containing der(1)t(1;18)(q10;q10), i(8)(q10), der(13)t(13;22)(q10;q10) and trisomy 20. The remaining population is generally made up of cells with non-clonal aberrations that were derived from the two major clones. Also, the non-clonal cell population may increase at high passages. | ||
Gender: | male | ||
Comments: | The Barrett's esophagus cell line, CP-A (also identified as KR-42421 or QhTERT), was derived from an endoscopic biopsy specimen obtained from a region of non-dysplastic metaplasia and transduced with the retroviral expression vector, pLXSN-hTERT, to create an immortalized cell line. This cell line shows increased telomerase activity and long telomeres of about 12 kb as assessed by terminal restriction fragment lengths (TRF) analysis. Morphologically, the cells are similar to early passage cultures: smaller cells with a larger nucleus to cytoplasm ratio. Genetic instability studies using flow cytometry and FISH reveal the retention of elevated tetraploidy (G2/tetraploidy) in the hTERT-immortalized cells, similar to the non-transduced parental cells. This well-characterized pre-malignant culture represents a unique opportunity to study factors that are important in both cancer progression and hTERT immortalization. [16173160] As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. In addition, it has been verified that no gross changes are observed in karyotype and morphology during the first 10 population doublings. |
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Propagation: | ATCC complete growth medium: The base medium for this cell line is MCDB-153. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:5 is recommended. Medium renewal: every 3 to 4 days |
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Preservation: | Freeze medium: RPMI-1640 Medium, 80%; fetal bovine serum, 10%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
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Doubling Time: | approximately 34 hours | ||
Related Products: | Recommended base medium for freezing (without the additional serum described under Freeze Medium): ATCC 30-2001 Recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X Erythrosin B vital stain solution: ATCC 30-2404 |
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References: | 16173160: Palanca-Wessels MC, et al. Genetic Analysis of Long-term Barrett's Esophagus Epithelial Cultures Exhibiting Cytogenetic and Ploidy Abnormalities. Gastroentrology 114:114-295, 1998. PubMed: 9453489 16173161: Palanca-Wessels MC, et al. Extended lifespan of Barrett's esophagus epithelium transduced with the human telomerase catalytic subunit: a useful in vitro model. Carcinogenesis 24(7): 1183-1190, 2003. PubMed: 12807723 16173615: Barrett MT, et al. Molecular Phenotype of Spontaneously Arising 4N (G2-Tetraploid) Intermediates of Neoplastic Progression in Barrett's Esophagus. Cancer Res. 63: 4211-4217, 2003. PubMed: 12874028 16173616: Maley CC, et al. Genetic clonal diversity predicts progression to esophageal adenocarcinoma. Nat. Genet. 38(4): 468-473, 2006. PubMed: 16565718 |
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