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- 详细信息
- 询价记录
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 器官来源：
直肠
- 库存：
大量
- 生长状态：
贴壁生长
- 相关疾病：
大肠癌
- ATCC Number：
CCL-234™
- 年限：
Dukes' type C
| Designations: | SW1463 [SW 1463, SW-1463] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
||
| Source: | Organ: rectum Tumor Stage: Dukes' type C Disease: colorectal adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen (CEA) 223 ng/10 exp6 cells/10 days; keratin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | blood type A; Rh + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11,12 D13S317: 12,13 D16S539: 11 D5S818: 13,14 D7S820: 9 THO1: 6,7 TPOX: 8,11 vWA: 16 |
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| Isoenzymes: | ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 1-2 |
||
| Age: | 66 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line was derived from a grade II mucous producing and grade III solid tumor. CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium Renewal: 1 to 2 times per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells. Resuspend in fresh medium and dispense into new flasks. Subculture just prior to confluency (about 10 to 12 days). |
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| References: | 23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501 | ||
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SW1463
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