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- 文献和实验
- 技术资料
- 年限：
75 years
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2175™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 器官来源：
肾脏
| Designations: | SW 156 [SW-156, SW156] | ||
| Depositors: | W McCombs | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: hypernephroma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432 | ||
| Antigen Expression: | Blood Type O; Rh + | ||
| Age: | 75 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The SW 156 line was established in 1972 by A. Leibovitz from a hypernephroma. | ||
| Propagation: | ATCC complete growth medium: Leibovitz's L-15 medium with 2mM L-glutamine, 90%; fetal bovine serum, 10% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |
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文献和实验Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation
are unclear. In this chapter, we describe a detailed method for overexpressing miR 156 in cotton using Agrobacterium -mediated genetic transformation. This provides an approach to investigate the function and regulatory mechanism of miRNAs in cotton.
藤红素和 19-048 治疗后能抑制结直肠癌细胞的增殖以及降低细胞活力。为了确定雷公藤红素和 19-048 是否通过靶向 PRDX1 影响细胞氧化还原状态，作者在 SW620 和 HCT116 细胞中用 DCFH-DA 荧光探针染色检测 ROS 水平，在不同浓度的雷公藤红素和 19-048 处理后，与对照相比，细胞中的 ROS 含量急剧升高，随后检测了雷公藤红素和 19-048 对 NCM460 细胞中 ROS 水平的影响，结果表明，雷公藤红素和 19-048 对正常细胞 ROS 诱导的影响小于癌
「外泌体 + 环状 RNA」国自然双热点助力肿瘤研究发表高分文章
的外泌体能促进 CRC 的增殖、迁移和侵袭 作者从两种 CRC 细胞系（HCT116和SW480）上清中分别分离获得外泌体，经过透射电镜、NTA 和 WB 鉴定后，确认已分离出外泌体。随后，作者用外泌体处理对应的 CRC 细胞，发现外泌体能显著促进 CRC 细胞增殖、迁移和侵袭，并减少凋亡。 WB 结果也显示，外泌体能提升 CRC 细胞中 BCL-2、 N-cadherin、Vimentin、MMP9 蛋白水平，和降低 E-cadherin、Cleaved-caspase3、Cleaved
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