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- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞形态：
上皮样
- 组织来源：
duct
- 相关疾病：
导管癌
- ATCC Number：
CRL-2320™
- 运输方式：
冻存运输
- 年限：
TNM stage IIA, grade 3
- 器官来源：
乳房
- 生长状态：
贴壁生长
- 库存：
大量
| Designations: | HCC1008 | ||
| Depositors: | AF Gazdar, AK Virmani | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast Tissue: duct Tumor Stage: TNM stage IIA, grade 3 Disease: ductal carcinoma Derived from metastatic site: lymph node |
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| Cellular Products: | Epithelial glycoprotein 2 (EGP2) cytokeratin 19 |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935. | ||
| Isolation: | Isolation date: June 7, 1994 | ||
| Applications: | The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR). The cells are positive for expressions of Her2-neu and p53 oncogenes. HCC1008 is positive for the epithelial cell specific marker, Epithelial Glycoprotein 2 (EGP2) and cytokeratin 19. |
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| Receptors: | estrogen receptor, not expressed progesterone receptor, not expressed |
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| Oncogene: | her2/neu +, p53 + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 14,15 D5S818: 13 D7S820: 10 THO1: 7 TPOX: 11 vWA: 17,19 |
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| Cytogenetic Analysis: | multiploid; cell population has several ploidy indices; double minute (DM) chromosomes were observed | ||
| Age: | 67 years adult | ||
| Gender: | female | ||
| Ethnicity: | Black | ||
| Comments: | This cell line was initiated from an axillary lymph node on 6/7/94 and took 12.5 months to establish. The tumor was classified as TNM stage IIA, grade 3, ductal carcinoma with 12 out of 12 lymph node metastasis. The cells are positive for expressions of Her2-neu and p53 oncogenes. HCC1008 is positive for the epithelial cell specific marker, Epithelial Glycoprotein 2 (EGP2) and cytokeratin 19. The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR). |
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| Propagation: | ATCC complete growth medium: HITES medium supplemented with 10% fetal bovine serum.The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium:
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: This cell line grows exceedingly slow. Cells grow in patches and cultures only become 50 to 60% confluent. |
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| Subculturing: | Protocol:
Cells reattach slowly after subculture (about 50% attached and 50% in suspension). Allow flasks to remain undisturbed for at least a week for cultures to become reestablished. It can take as along as three to four weeks before cultures can be subcultured again. Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Every 3 to 4 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 Medium additive:ATCC 30-2214 normal (or near-normal) cell line established from the same patient:ATCC CRL-2319 purified DNA:ATCC CRL-2320D purified RNA:ATCC CRL-2320R |
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| References: | 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 | ||
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文献和实验相关实验
Direct DNA Injection (p53) into HCC Tumors
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文献速递：全面液体分析循环肿瘤DNA和蛋白质生物标志物为评估预后带来新的可能
研究背景 肝细胞癌（HCC）被认为是中国癌症相关死亡率的第三大原因。尽管手术是治疗HCC的较佳选择，但由于缺乏早期症状，大多数患者在癌症晚期才被诊断出来，因此肿瘤复发的风险很高。识别可能复发的患者并给予对应的辅助治疗，仍然是一个临床难题。循环肿瘤DNA（ctDNA），也可称为肿瘤衍生的细胞游离DNA，包含有关肿瘤基因组图谱的全面信息，包括单核苷酸变异（SNVs）、拷贝数变异（CNVs）和表观遗传变异。将ctDNA与HCC中的传统血清蛋白生物标志物结合将为无创监测实时肿瘤进展提供新的可能。
学问题。 案例一：多组学分析揭示肝癌起始细胞免疫逃逸机制和精准治疗策略 发表期刊：Cancer Cell 影响因子：44.5 发表时间：2024 年 12 月 研究疾病：肝细胞癌（Hepatocellular Carcinoma,HCC） 样本类型：患者来源的肝癌类器官（HCC organoids），肝癌细胞系（Hep3B,Huh7,PLC/PRF/5, MHCC97 H,Hep53.4）和小鼠模型（包括自发性肝癌模型和异种移植模型） 样本数量：11 个 HCC 类器官样本，4 个肝癌细胞系样本
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HCC1008
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