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- 相关疾病：
淋巴瘤
- ATCC Number：
HTB-176™
- 库存：
大量
- 年限：
53 years
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 细胞类型：
其他细胞类型
- 细胞形态：
淋巴样
| Designations: | H9 [derivative of HuT 78] | ||
| Depositors: | RC Gallo, M Popovic | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Disease: lymphoma Cell Type: cutaneous T lymphocyte; |
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| Cellular Products: | interleukin-2 (interleukin 2, IL-2) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Receptors: | interleukin 2 (IL-2) | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | CD4; HLA A1, B62, C3, DR4, DQ3 | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11 D13S317: 8,12 D16S539: 11,12 D5S818: 11 D7S820: 8,11 THO1: 8,9 TPOX: 8,9 vWA: 14,15 |
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| Cytogenetic Analysis: | This is a near triploid cell line (modal number = 69; range = 58 to 74). The frequency of higher ploidies is 2.5%. The line has an extremely complex karyotype with nearly 60% of the chromosomes in each cell being structurally altered marker chromosomes., Among the markers are t(3p4q), t(5q6q), t(5p6p), i(18q), i(18p); t(4q7p), and del(7)(q32). The first four of these are usually paired. Normal N4, N5, N6, N7, N10, N13, N18, N19, N20 an X are absent. | ||
| Isoenzymes: | AK-1, 0 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 0 PGM1, 1 PGM3, 0 |
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| Age: | 53 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The H9 cell line is a clonal derivative of the Hut 78 cell line (see ATCC TIB-161 ). The H9 clone was selected for permissiveness for HIV-1 replication, and has been used to isolate and propagate HIV-1 from the blood of patients with acquired immunodeficiency syndrome (AIDS) and pre-AIDS conditions. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml. Do not allow cell concentration to exceed 3 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 parental cell line:ATCC TIB-161 |
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| References: | 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177 22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 22948: Popovic M, et al. Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and pre-AIDS. Science 224: 497-500, 1984. PubMed: 6200935 23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674 23367: Popovic M, et al. T4 positive human neoplastic cell lines susceptible to and permissive for HTLV-III. Lancet 2: 1472-1473, 1984. PubMed: 6151082 29139: Brigino E, et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366 32558: Anderson SM, et al. Intercellular transfer of a glycoslphosphatidylinositol (GPI)-linked protein: release and uptake of CD4-GPI from recombinant adeno-associated virus-transducted HeLa cells. Proc. Natl. Acad. Sci. USA 93: 5894-5898, 1996. PubMed: 8650189 |
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Protective antibody responses against HIV-1 have yet to be identified or determined. HIV-1 envelope gpl20/gp41 is known to exist as a multimer (tetramers or trimers) on the surface of the virion (1 –4 ). A number of immunoassays
首先，它肯定不同于原代细胞系的，最大的区别，原代的心肌细胞基本不能分裂，而H9C2可以分裂，最大的区别了吧！原代心肌细胞会跳动，你的H9C2不会吧。虽然都是梭形的，但是H9C2来源于胚胎期心脏，而心肌细胞一般用新生大鼠分离获得。H9C2与原代心肌细胞有那么大区别，当然不能替代它，所以在研究方向上，你要根据自己的实验需求选择自己合适的细胞，一般来讲呢，如果你研究与心脏相关的信号通路，我建议你还是选用细胞系，因为它可以分裂，获得细胞容易，而且，因为H9C2展现一些成肌细胞特性，所以一般文章都说H9
these processes based on spectrophotometric analysis. The application of simple, rapid, and sensitive fluorescence methods to determine the cytotoxicity and COS is described in the present chapter. Murine H9c2 cells were exposed to various free radical and non
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