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- 细胞形态：
上皮样
- 相关疾病：
大肠癌
- 器官来源：
结肠
- ATCC Number：
CL-187™
- 年限：
Dukes' type B
- 库存：
大量
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
冻存运输
| Designations: | LS 180 | ||
| Depositors: | Northwestern University | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Tumor Stage: Dukes' type B Disease: colorectal adenocarcinoma |
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| Cellular Products: | carcinoembryonic antigen interleukin 10 interleukin 6 mucin |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood type O |
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| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,13 D13S317: 10 D16S539: 11,13 D5S818: 10,15 D7S820: 11,9.3 THO1: 6,7 TPOX: 8,9 vWA: 15,18 |
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| Cytogenetic Analysis: | modal number = 45; range = 42 to 47. The stemline karyotype was 45,t(X;5) (q12;q31) with tetraploid-like cells occurring at 2.2 percent. Occasional changes in karyotypes were also noted as a result of balanced translocations producing new marker chromosomes. In conjunction with this, decreases in chromosomes involving interchromosomal exchanges were seen. The karyotype was exactly the same as that originally described by the depositor. |
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| Isoenzymes: | ADA, 1 ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
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| Age: | 58 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | This line has never been trypsinized; however, the variant LS 174T (ATCC CL-188 ) has been subcultured by trypsinization. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove most medium. Dislodge cells from the floor of the flask by banging flask, washing cells by repeat pipetting and scraping gently. Aspirate and dispense into new flasks. These cells have never been trypsinized. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: Two to three times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 derived from same individual:ATCC CL-188 |
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| References: | 2148: Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041 3524: Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980 22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228 32532: Schuetz EG, et al. P-glycoprotein: a major determinant of rifampicin-inducible expression of cytochrome P4503A in mice and humans. Proc. Natl. Acad. Sci. USA 93: 4001-4005, 1996. PubMed: 8633005 |
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【求助】180KD的膜蛋白western转膜条件怎样？（湿转）
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chuenshui 细胞株：LS 174T 来源：中科院上海细胞库 培养基 :DMEM 10%小牛血清 问题：刚买回来时传代2次，都没问题。扩大培养时发现，细胞养起来时培养基发紫，0.25%胰酶消化传代，24小时候细胞50%不贴壁，细胞还呈发亮状态。 尝试解决办法：更换培养基，10小时后培养基发紫，细胞依然呈漂浮状态。无解决现象。 请问有什么办法可以解决，培养箱应该没问题，箱子里有同时培养的其他细胞，无异
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LS 180
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