C32产品图

C32

收藏
  • 询价
  • 2026年09月29日
    avatar
    15 年金牌会员
  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 运输方式:

      冻存运输

    • 相关疾病:

      其他疾病

    • 器官来源:

      皮肤

    • ATCC Number:

      CRL-1585™

    • 年限:

      53 year old adult

    • 物种来源:

      人

    • 是否是肿瘤细胞:

      1

    • 库存:

      大量

    • 生长状态:

      贴壁生长

    Designations: C32
    Depositors:  TR Chen
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology:
    Source: Organ: skin
    Disease: melanoma, amelanotic
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Tumorigenic: Yes
    DNA Profile (STR): Amelogenin: X,Y
    CSF1PO: 11,12
    D13S317: 12
    D16S539: 11,12
    D5S818: 12
    D7S820: 9,11
    THO1: 9.3
    TPOX: 9
    vWA: 14,19
    Age: 53 year old adult
    Gender: male
    Ethnicity: Caucasian
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37C.

    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: Twice per week
    Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
    recommended serum:ATCC 30-2020
    derivative:ATCC CRL-1579
    0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
    Cell culture tested DMSO:ATCC 4-X
    References: 26091: Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716
    26092: Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验
    • 高度内标法(单组分单点二次校正)

        例:标准样品的配制:精密称取98.54mg VE标准品(含量为99.3%)于100ml容量瓶,用含1mg/ml内标物C32的正已烷稀释至刻度。可算出VE标准品的浓度(即标浓度1)为0.9785mg/ml; 样品1的配制:精密称取96.13mg 样品于100ml容量瓶,用含1mg/ml内标物C32的正已烷稀释至刻度。可算出VE样品的浓度(即样品量)为0.9613mg/ml 样品2的配制:精密称取90.10mg 样品于100ml容量瓶,用含1mg/ml内标物C32的正已烷

    •   代理SMC气缸#SMC气缸故障维修信息

      CD55B32-5 CD55L32-5 CD55F32-5 CD55G32-5 CD55C32-5 CD55B32-10 CD55L32-10 CD55F32-10 CD55G32-10 CD55C32-10 CD55B32-15 CD

    • 利链菌素 streptolydigin

      从放线菌Streptomyces lydicus的培养液中得到的抗菌素。 C32 H44 N2 O9 在抑制细菌的 RNA聚合酶方面有与利福霉素( rifamycin)相似的作用,即阻止 RNA链的延长。  

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    询价
    ATCC细胞库
    2026年09月29日询价
    ¥500
    北京百奥创新科技有限公司
    2025年07月11日询价
    ¥4000
    上海格宁生物科技有限公司
    2026年08月29日询价
    ¥680
    上海联迈生物工程有限公司
    2026年05月13日询价
    ¥800
    上海康朗生物科技有限公司
    2025年07月15日询价
    C32
    询价