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- 文献和实验
- 技术资料
- 年限：
53 adult
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1579™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 库存：
大量
- 器官来源：
皮肤
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | C32TG [C32-r16TG] | ||
| Depositors: | TR Chen | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: skin Disease: melanoma, amelanotic |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12 D16S539: 11,12 D5S818: 12 D7S820: 9,11 THO1: 9.3 TPOX: 9 vWA: 14,19 |
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| Age: | 53 adult | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The cells are resistant to 6-thioguanine and sensitive to HAT medium. This line was derived from C32 (ATCC CRL-1585 ) by selection for 6-thioquanune resistance. They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-). |
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| Propagation: | ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10%; 0.050 mM 6-thioguanine |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution several minutes until cells detach. Add fresh medium and centrifuge. Resuspend in fresh medium and dispense into new flasks. |
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| Related Products: | derivative:ATCC CRL-1585 | ||
| References: | 1082: Chen TR. Chromosome changes in 6-TG-resistant mutant strains derived from a karyotypically stable human line, C32. Cytogenet. Cell Genet. 35: 181-189, 1983. PubMed: 6861522 26091: Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716 26092: Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368 |
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Transgenic Mouse and Gene Targeting Facility Tg 008 Southern Blotting Materials and Equipment Hybaid hybridization oven (ISC BioExpress, H-9250) Vacuum oven (VWR, 52201-218) Ludlum Geiger Counter Radiation Shields
APP-Based Transgenic Models: The Tg2576 Model
, economic, and personal impact. In 1996, Karen Hsiao and colleagues showed that transgenic mice, which overexpress a human APP cDNA transgene from a Swedish family whose members showed early onset, familial AD (Tg2576 mice) were cognitively impaired
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