Tg 008 Southern Blotting
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1790
Transgenic Mouse and Gene Targeting Facility
Tg 008 Southern Blotting
Materials and Equipment
Hybaid hybridization oven (ISC BioExpress,H-9250)
Vacuum oven (VWR,52201-218)
Ludlum Geiger Counter
Radiation Shields and lock box (from various vendors)
Mini-centrifuge (VWR,20668-212)
Thermolyne Dri-Bath,Type 17600 (VWR,35756-006)
Platform Shaker,New Brunswick Scientific (VWR,40000-300)
Electrophoresis Separation Systems (Owl)
QIAquick Nucleotide Removal Kit (Qiagen,28304)
Prime-It II Random Primer Labeling Kit (Stratagene,300385)
[alpha-32P]dCTP (ICN)
Salmon Sperm DNA (Stratagene,201190)
SDS solution,20% w/v (Bio-Rad,161-0418)
Zeta-Probe Genomic Tested Blotting Membrane (nylon)(Bio-Rad,162-0196)
Gel Blot Paper GB002 (Schleicher
