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- 询价记录
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 器官来源：
膀胱
- 年限：
grade 3
- 库存：
大量
- 相关疾病：
肿瘤
- 运输方式：
冻存运输
- ATCC Number：
CRL-1472™
- 细胞形态：
上皮样
| Designations: | HT-1376 | ||
| Depositors: | S Rasheed | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: urinary bladder Tumor Stage: grade 3 Disease: carcinoma |
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| Cellular Products: | fibrinolytic activity; interferon | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| Reverse Transcript: | negative | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 9,11 D16S539: 11,14 D5S818: 11,12 D7S820: 9,12 THO1: 7,10 TPOX: 8 vWA: 15,18 |
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| Cytogenetic Analysis: | modal chromosome number = 104 to 121 | ||
| Isoenzymes: | G6PD, B | ||
| Age: | 58 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The line does not contain detectable virus particles, herpesvirus antigens or retrovirus antigens. The cells will grow in soft agar. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow culture to sit at room temperature or 37C until cells detach (about 10 minutes), add fresh medium, aspirate and dispense into new flasks. |
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| Related Products: | purified DNA:ATCC CRL-1472D | ||
| References: | 22538: Rasheed S, et al. Human bladder carcinoma: characterization of two new tumor cell lines and search for tumor viruses. J. Natl. Cancer Inst. 58: 881-890, 1977. PubMed: 191628 23134: Jameson P, Grossberg SE. Production of interferon by human tumor cell lines. Arch. Virol. 62: 209-219, 1979. PubMed: 229805 25065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 |
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文献和实验小鼠5羟色胺(5-HT) 酶联免疫 分析（ELISA） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 5羟色胺(5-HT)的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 5羟色胺 (5-HT) 水平。用纯化的 小鼠 5羟色胺 (5-HT) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 5羟色胺 (5-HT) ， 再与HRP 标记
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
assays for measuring the binding of radioligands to two major types of receptors for 5?hydroxytryptamine (5?HT or serotonin), 5?HT1 and 5?HT2 receptors, in homogenates of brain tissue or cloned into cells in culture. The specific receptor subtypes covered
做好准备工作之后，先把旧的培养基倒掉。再用PBS洗两遍，然后加0.05%胰酶/EDTA，剂量根据培养瓶大小定，一般是25cm 2 的瓶子大约0.8-1ml，轻摇10——15秒，使消化液均匀覆盖瓶底即可，再倒掉。置培养箱3-5分钟(看消化效果而定)，等大部分细胞呈流沙状滑落时，即加入培养基终止消化，一般以1:5左右的比例传代(视计数结果和看细胞长满的程度而定)。这种方法养比较顽强的细胞很好，既节省步骤，也降低了离心和较长时间消化对细胞带来的损伤，其实大家也可以试试用这种方法养别的细胞。主要做
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