AccB7I内切酶价格

特别提示：包括AccB7I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：AccB7I内切酶
英文名称：AccB7I
产品货号：GS2144
产品规格：200U

储存条件：冷冻（-20℃）

使用说明

High enzyme concentration may result in star activity.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dcm-)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	CCANNNN↑NTGG
来源	Acinetobacter calcoaceticus B7
Assayed on	Lambda DNA(dcm-)
Optimal buffer	G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Blocked by overlapping Dcm methylation(CmCWGG):CCANNNCCTGG or CCAGGNNNTGG.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	BasI,PflBI,PflMI,Van91I

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