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- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 相关疾病：
腺癌
- ATCC Number：
CRL-1687™
- 库存：
大量
- 年限：
61 years
- 器官来源：
胰腺
| Designations: | BxPC-3 | ||
| Depositors: | MH Tan | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: pancreas Disease: adenocarcinoma |
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| Cellular Products: | mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 9,11 D5S818: 11 D7S820: 10,13 THO1: 9 TPOX: 8 vWA: 14,18 |
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| Age: | 61 years | ||
| Gender: | female | ||
| Comments: | The cells do not express the cystic fibrosis transmembrane conductance regulator (CFTR). A CFTR positive pancreatic line, Capan-1 is available (ATCC HTB-79 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 22737: Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226 23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 23153: Chambers JA, Harris A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993. PubMed: 7691840 58045: Tan MH, et al. Characterization of a new primary human pancreatic tumor line. Cancer Invest. 4: 15-23, 1986. PubMed: 3754176 |
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文献和实验to establish that antibodies targeting the epidermal growth factor receptor and vascular endothelial growth factor receptor-2 are synergistic with regard to antitumor effects, in a BxPC-3 subcutaneous xenograft model for pancreatic cancer.
with BxPC-3 xenografts using fluorouracil, cisplatin, and doxorubicin with a consequent size reduction of the tumors between 72% and 79%, compared to the controls at day 28 after the first treatment. Although these figures are impressive, by any standard
610A 采用 CytoFLEX 流式细胞仪上的紫光侧向散射光，有利于进行亚微颗粒分析和计数
（其他荧光微球， 例如，B.D.FACS & LSR、Apogee A50、Stratedigm、MacsQuant、… 1）。Gigamix = 两种 Mgx+ 的 v/v 混合物。其他荧光 75 nm 微球。细胞源的微颗粒：无血小板血浆中的 PMP (CD41/AnnV)（正 常 供 体）；TF+ MP (SBTF1-PE/AnnV-F) & EPC-MP (CD59-PE/AnnV-F)，从 BxPC3 胰腺癌细胞和血管内皮祖细胞培养液中纯化并提取出来。结果1. CytoFLEX 采用 SSC
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