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![【ATCC来源】MCF7 [MCF7]、 [MCF-7]细胞、MCF7 [MCF7]细胞、MCF7 [MCF-7]MCF7 [MCF7][人乳腺癌细胞]](https://img1.dxycdn.com/2021/0721/054/1466071650690953943-14.jpg!wh200)
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- 文献和实验
- 技术资料
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1718™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 生长状态：
贴壁生长
- 器官来源：
大脑
- 年限：
grade IV
- 库存：
大量
- 运输方式：
冻存运输
- 细胞形态：
其他
| Designations: | CCF-STTG1 | ||
| Depositors: | BP Barna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | astrocytic |
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| Source: | Organ: brain Tumor Stage: grade IV Disease: astrocytoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | HLA-DR; Homo sapiens, expressed | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 11,13 D16S539: 11,12 D5S818: 12,13 D7S820: 10,11 THO1: 7,8 TPOX: 8,11 vWA: 17 |
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| Cytogenetic Analysis: | This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired. | ||
| Age: | 68 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | The CCF-STTG1 cell line was established from a specimen of Grade IV astrocytoma from a 68-year-old Caucasian female by mincing and culturing in RPMI 1640 with 10% FBS. The cells exhibit strong perinuclear acid phosphatase activity and glial fibrillary acidic protein is present in 70% to 80% of the cells. HLA DR is expressed on 20% to 30% of the cells after 48 hours in culture. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 21868: . Experimental allergic encephalitis. New York: Liss; 1984. 22187: . . In Vitro 19: 275, 1983. 22223: . . Fed. Proc. 43: 781, 1984. 22881: Barna BP, et al. Enhanced DNA synthesis of human glial cells exposed to human leukocyte products. J. Neuroimmunol. 10: 151-158, 1985. PubMed: 3877740 |
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