![NG108-15 [108CC15]](https://custom.dxycdn.com/trademd/upload/pic/2012/03/29/1332499969.jpg!small.t.1)
- 询价
- 2025年09月12日
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企业认证
- 详细信息
- 技术资料
- 器官来源：
大脑
- 库存：
大量
- 细胞形态：
其他
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 相关疾病：
其他疾病
- ATCC Number：
HB-12317™
| Designations: | NG108-15 [108CC15] | ||
| Depositors: | Univ. Texas Southwestern Medical Cntr. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus (neuroblastoma); Rattus norvegicus (glioma) deposited as mouse (neuroblastoma); rat (glioma) | ||
| Morphology: | flat; round; 10 to 100 micrometers diameter |
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| Source: | Organ: brain Disease: glioblastoma; neuroblastoma Cell Type: somatic cell hybrid |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Comments: | The NG108-15 cell line, originally named 108CC15, was developed in 1971 by Bernd Hamprecht. [112482 ] The line was formed by fusing mouse N18TG2 neuroblastoma cells with rat C6-BU-1 glioma cells in the presence of inactivated Sendai virus. [51493 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium (GIBCO/InVitrogen Catalog No.12100-061, DMEM without sodium pyruvate ). To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 51492: Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int. Rev. Cytol. 49: 99-170, 1977. PubMed: 16829 51493: Hamprecht B, et al. Culture and characteristics of hormone-responsive neuroblastoma X glioma hybrid cells. Methods Enzymol. 109: 316-341, 1985. PubMed: 2985920 112482: Bernd Hamprecht, personal communication |
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NG108-15 [108CC15]
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