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- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 库存：
大量
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- ATCC Number：
CRL-1768™
| Designations: | HP6050 | ||
| Depositors: | CB Reimer | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human IgG3 hinge region | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the Centers for Disease Control, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Hybridoma 3:263-275, 1984). | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with purified human myeloma protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with hinge region epitopes that are different from those reactive with HP6047 (ATCC CRL-1774 ). Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 58066: Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 58067: Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923 |
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文献和实验惠普（HP）是一家立足于进步和创新的高科技企业。作为惠普（HP）的核心研究机构，惠普实验室担负着开创公司未来的重任。 惠普实验室的职责 惠普的大多数研究工作都有其业务集团主导，主要是开发各种产品和服务，以满足客户需求。 惠普实验室的职责是开发新的突破性技术和技术进步，为惠普（HP）增加竞争优势，并在惠普（HP）现有的策略之外创造新的商机。 同时，实验室还负责惠普公司（HP）的战略制定和调整，并投资于惠普关注的基础科学和技术领域。
真空箱DZF-6050调试方法 0512-69353538 抽真空调试: a)将箱门关上并将门拉手旋紧到位，关闭放气阀(使橡皮塞上的孔与放气阀上的孔扭偏90°),开启真空阀(由逆时针旋转90°)，第一次使用可能真空阀开关较紧，可用力旋转。 b)用随机配件真空连接管(内径:Φ16mm壁厚:10mm)将真空干燥箱抽气管(外径:Φ16mm)和真空泵(2XZ-2型，进气口外径Φ16mm)连接牢固(6090及6210型已连接好)。接通真空
（1）原理： 在待测血清中加入一定量的血红蛋白液，使之与待测血清中的结合珠蛋白（Hp）形成Hp-Hb复合物。通过电泳法将结合的Hp-Hb复合物与未结合的Hb分开，测定Hp-Hb复合物的量，从而得到血清中结合珠蛋白的含量。 参考值：0.8～2.7g/L. （2）临床意义： 增高见于妊娠、慢性感染、恶性肿瘤等，但不能排除溶血；减低见于各种溶血、肝病或无结合珠蛋白血症、巨幼细胞贫血等。
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