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- 文献和实验
- 技术资料
- 库存：
大量
- 生长状态：
贴壁生长
- 品系：
C57BL/KaLw
- 细胞形态：
成纤维样
- ATCC Number：
CCL-63™
- 器官来源：
胚胎
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 年限：
embryo
| Designations: | NCTC 4093 | ||
| Depositors: | KK Sanford | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Strain: C57BL/KaLw |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Virus Resistance: | poliovirus 1 | ||
| Antigen Expression: | H-2d | ||
| Age: | embryo | ||
| Comments: | 10% horse serum may be used instead of fetal bovine serum in medium. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: NCTC 135 medium, 90%; fetal bovine serum, 10%; adjust pH to circa 7.2 with humidified 5 to 10% CO2 in air |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Subcultures can be prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Alternatively, a standard trypsinization procedure may be used. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 26218: Kampine JP, et al. Diagnosis of gaucher's disease and niemann-pick disease with small samples of venous blood. Science 155: 86-88, 1967. PubMed: 6015567 | ||
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文献和实验相间（光强）的变化，这种变化利用光电传感器（光敏电阻）很容易转换成电脉冲信号记录下来。 仪器描述 模拟光电型血细胞计数器由光源、光敏电阻、施密特电路、示波器组成。见图一。 图一、模拟光电型血细胞计数器的基本部件 实验电路如图二所示。让光线照射在光敏电阻上，让一张黑纸遮住光线（模拟有血细胞经过其间），则在光敏电阻两端就会产生电压变化。每当用黑纸遮挡一次，就相当于有一个血细胞经过，会在光敏电阻上产生一个脉冲，脉冲总的个数就反映了血细胞数目的多少。脉冲信号经过CD4093
to each flask. Tip, then bang. Add 20% FBS NCTC media and tilt. 4 ml per flask. Scrape (most of the small cells are already released; proliferative cells lift up easy, differentiative cells are more adherent). Place contents into a 50 ml Falcon tube
Testing for Bacteria and Fungi
ml aliquots) (Prod. No. S1674 ) TSB Powder (Prod. No. T8907 ) Fluid Thioglycollate Medium (20ml aliquots) (TGM) (Prod. No. F4797 ) Bacillus subtilis NCTC* Candida albicans NCTC* Clostridium sporogenes NCTC* Equipment
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