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- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- ATCC Number：
HB-8785™
- 细胞形态：
淋巴样
| Designations: | F3B6 | ||
| Depositors: | Cetus Corp. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | human (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Fusion of human peripheral blood B cell with NS-1 myeloma cells. The cells are resistant to 6-thioguanine. This line is suitable for fusing to human B cells for the production of human monoclonal antibodies. |
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| Propagation: | ATCC complete growth medium: HL-1 medium with 2 mM L-glutamine, 99%; fetal bovine serum, 1%. HL-1 medium can be obtained from Lonza (catalog number 77201). |
||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 1 X 10 exp6 cells/ml. |
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| Preservation: | HL-1 medium, 82.5%, human serum albumin, 10%; DMSO, 7.5% | ||
| References: | 3598: Larrick JW, Raubitschek AA. Pseudomonas aeruginosa exotoxin A antibodies, their preparation and use. US Patent 4,677,070 dated Jun 30 1987 | ||
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文献和实验ROTORS Table F.1. Rotor Characteristics Figure F.1. Cross section of Sorvall SS-34 rotor. Rotors for a centrifuge are either fixed angles , swinging buckets , continuous flow, or zonal, depending upon whether the sample is held
P＝0.05 （双侧） r r（较大均方的自由度） n 2 2 3 4 5 6 7 8 9 10 12 15 20 30 60 00 1 799 364 899 922 937 948 957 963 969 977 985 993 1001 1010 1018 1 2 39.0 39.2 39.2 39.3 39.3 39.3 39.4 39.4 39.4 39.4 39.4 39.4 39.5 39.5 39.5 2 3 10.0 15.4 15
指在大肠杆菌等细菌细胞中具有能和染色体分开进行独立复制的 F因子的雄性菌株。具有 F纤毛，通过与雌性菌株接合，可以高的频率进行 F因子的传递，同时也以低频率雄性菌的染色体向雌性菌传递。 F 菌株群体中有时有的细菌会失去 F因子而变为 F- 菌，但这种频率是很低的（约为 0.1%）。通过在含有适当浓度的吖啶色素的培养基中培养等方法，由于仅 F因子的复制受特异的抑制，所以可以很高的频率获得 F-菌株。
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