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- 文献和实验
- 技术资料
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- ATCC Number：
HB-9463™
- 库存：
大量
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
| Designations: | F21-1D3G7C8 | ||
| Depositors: | Cornell Res. Fndn., Inc. | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against Agrobacterium tumefaciens biovar 3 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with heat-inactivated Agrobacterium tumefaciens biovar 3 strain CG-49 (ATCC 53691 ). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for Agrobacterium tumefaciens biovar 3 (the pathogen associated with grapevine disease) and can be used to diagnose grapevine disease. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1.5 mM HEPES and 0.1 mM non-essential amino acids, 80%; fetal bovine serum, 20% Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 5295: Burr TJ, et al. Monoclonal antibody and method for diagnosing grape disease employing same. US Patent 5,037,755 dated Aug 6 1991 22693: Bishop AL, et al. A monoclonal antibody specific to Agrobacterium tumefaciens biovar 3 and its utilization for indexing grapevine propagation material. Phytopathology 79: 995-998, 1989. |
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文献和实验The STINT‐NMR Method for Studying In‐cell Protein‐Protein Interactions
onto the three‐dimensional structure of ubiquitin (PDB code 1D3Z). Individual residues exhibiting either a chemical shift change >0.05 ppm or differential broadening are indicated in red. All perturbed residues lie on the ubiquitin surface and, therefore, reflect changes
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