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- 细胞形态：
成纤维样
- 相关疾病：
其他疾病
- ATCC Number：
CCL-146™
- 组织来源：
connective tissue
- 年限：
14 months
- 库存：
大量
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | IMR-33 | ||
| Depositors: | JJ Tumilowicz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Meriones unguiculatus | ||
| Morphology: | fibroblast |
||
| Source: | Tissue: connective tissue Disease: fibroma |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Virus Resistance: | poliovirus 1; echoviruses | ||
| Age: | 14 months | ||
| Gender: | male | ||
| Propagation: | ATCC complete growth medium: Medium 199 with Hanks' BSS, 80%; fetal bovine serum, 20% |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 3 to 4 days Remove medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let culture sit for 10 to 15 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
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| References: | 23530: Tumilowicz JJ, Cholon JJ. Intracisternal type A particles and properties of a continuous cell line originating from a gerbil fibroma. Proc. Soc. Exp. Biol. Med. 136: 1107-1110, 1971. PubMed: 5554453 | ||
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