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- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-2347™
- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
| Designations: | IgG-9D5 | ||
| Depositors: | JL Goldstein, YK Ho | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against SCAP (SREBP [sterol regulatory element binding protein] cleavage activating protein) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The hybridoma cell line IgG-9D5 secretes a mouse monoclonal antibody (IgG2b) that recognizes hamster SCAP (SREBP [sterol regulatory element binding protein] cleavage-activating protein.) SCAP regulates cholesterol metabolism by stimulating cleavage of the transcription factors SREBP-1 and SREBP-2 in cultured mammalian cells. The antibody is useful in immunoblot and immunoprecipitation of SCAP in cell extracts from cells grown in different conditions; the results help to understand the domain interactions between SREBP and SCAP. The line was produced by fusing Sp2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized against a fusion protein encoding 6 consecutive histidines followed by amino acids 540-707 of hamster SCAP. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 4535: Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195 38220: Sakai J, et al. Identification of complexes between the COOH-terminal domains of sterol regulatory element-binding proteins (SHEBPs) and SREBP cleavage-activating protein. J. Biol. Chem. 272: 20213-20221, 1997. PubMed: 9242699 |
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