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- 文献和实验
- 技术资料
- 运输方式:
冻存运输
- 生长状态:
悬浮生长
- 库存:
大量
- ATCC Number:
CRL-1606™
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 免疫类型:
IgG1
- 细胞形态:
淋巴样
- 器官来源:
脾
| Designations: | HFN 7.1 | ||
| Depositors: | RC Schoen, KL Bentley, RJ Klebe | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Organ: spleen Cell Type: hybridoma: B lymphocyte; |
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| Cellular Products: | immunoglobulin; monoclonal antibody; against human fibronectin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2d | ||
| Comments: | The antibody does not cross-react with other fibronectins tested (26 species including 15 primates). Mice were immunized with purified human fibronectin. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). Formerly ATCC HB-39. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml. |
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| References: | 57999: Schoen RC, et al. Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125 | ||
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文献和实验相关实验
( 2 )碳化物 如: SiC , B4 C , WC , TiC , ZrC , HfC 等,它们是几类中熔点最高的,硬度大,但脆性也强。 ( 3 )氮化物 如: BN , Si3 N4 以及 AlN , ZrN , HfN 等,一般说氮化物是最硬的材料。 ( 4 )硼化物 如: ZrB2 , HfB2 , WB 和 MoB 等,熔点在 2000℃ 以上,它们的抗氧化性能最强。
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HFN 7.1
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