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- 相关疾病：
非小细胞肺癌
- ATCC Number：
CRL-5930™
- 生长状态：
贴壁生长
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
肺
- 运输方式：
冻存运输
| Designations: | NCI-H2172 [H2172] | ||
| Depositors: | AF Gazdar, JD Minna | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: lung Disease: non-small cell lung cancer |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233. | ||
| Gender: | female | ||
| Comments: | The line was established in May 1989. The tissue donor was a non-smoker. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37.0°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 23570: . NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.. | ||
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文献和实验2017 年 8 月 31 日，山东烟台毓璜顶医院呼吸内科邹慎春为第一作者和通讯作者 Yao Zhang 在《分子医学报告》（Molecular Medicine Reports）发表题为《Epicatechin induces apoptotic and autophagic cell death in NCI-H2172 non‑ small cell lung cancer cells along with concomitant mitochondrial membrane
位于小鼠第 17对染色体（第 1X连锁群）上，是决定小鼠主要组织亲和性抗原 H-2抗原表达的基因。一个小鼠个体的 H-2抗原包括许多抗原的复合体，它至少和 H-2K、 H-2G、 H-2D三个基因座位有关。 H- 2K和 H-2D位于两端，它们之间的交换率为 0.5％。由于在 H-2基因座位内包含有免疫应答基因（ Ir）、补体第四成分基因（ Ss）、雄特有蛋白质基因（ Slp）等，因此 H-2基因又称为 H-2复合体（ H-2 complex）（见图）。此外已经明确在哺乳动物的精子、桑椹
相关专题 （一）材料及试剂 1．培养基 RPMI-1640 或TC199培养液 2．PHA 同形态学方法 3．小牛血清 4．3H-TdR 选用比活性为2Mci~10Mci/mg分子 的制品，将1 Mci/ml溶液以生理盐水稀释成100µci/ml，于4℃保存备用。临用时再用培养液稀释成10µci/ml溶液。 5．3%冰醋酸 6．浓甲醛 7．30%H2O2液 8．闪烁液 PPO（2，5二苯基噁唑
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