- 询价
- 2026年02月09日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgE (Iga haplotype)
- 细胞形态：
淋巴样
- ATCC Number：
TIB-142™
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | IGEL a2 [15.3] | ||
| Depositors: | M Wabl | ||
| Isotype: | IgE (Iga haplotype) | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with TNP-Asc. Tested and found negative for ectromelia virus (mousepox). |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
||
| References: | 924: Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验人 CYP1A2酶联免疫 分析 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 CYP1A2 的含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 CYP1A2 水平。用纯化的 人 CYP1A2 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 CYP1A2 ，再与HRP 标记的 CYP1A2 抗体结合，形成抗体- 抗原 - 酶标抗体复合物 ，经过彻底
人膜联蛋白-A2(Anxa2) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中膜联蛋白-A2(Anxa2) 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人膜联蛋白 -A2(Anxa2) 水平。用纯化的人膜联蛋白 -A2(Anxa2) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入膜联蛋白
大鼠腺苷 A2b 受体 (A2b R)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 A2b R 单抗包被于酶标板上，标准品和样品中的 A2b R 与单抗结合，加入生物素化的抗大鼠 A2b R ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝色，最后加终止液硫酸
技术资料暂无技术资料 索取技术资料
