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- 文献和实验
- 技术资料
- 库存:
大量
- 物种来源:
大鼠
- 是否是肿瘤细胞:
0
- 器官来源:
甲状腺
- 细胞类型:
其他细胞类型
- 生长状态:
贴壁生长
- 运输方式:
冻存运输
- 相关疾病:
其他疾病
- ATCC Number:
CRL-1607™
| Designations: | 6-23 (Clone 6) | ||
| Depositors: | R Gagel, FN Zeytinoglu | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | |
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| Source: | Organ: thyroid Disease: medullary thyroid carcinoma Cell Type: C cell; |
||
| Cellular Products: | neurotensin; calcitonin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines. | ||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15% |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended Medium Renewal: 3 times per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 ml of 0.25% trypsin and let the culture sit at 37C for 6 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 12 days. |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| References: | 1085: Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326 1088: Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046 |
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文献和实验该词来源于希腊文的 Kλωγ,原义为小枝之集聚,但后来则指由无性繁殖所产生的同基因型的生物群,这是于 1903年由 H. J. Webber最早应用于生物学上的。过去曾译作具有繁殖意思的营养系或含有原语源意思的分支系(群),而现在则多按其原音直接称为克隆。现在该词在个体、细胞和基因等三方面都使用,而且任何一方面似乎都意味着来自同源的犹如复制一样的同一生物群体。( 1)关于个体:由无性繁殖所增殖的种群均是克隆,这在植物方面,通过体细胞培养,在试管内繁殖、分化可以重新生出完整
你那里天气怎么样?柚子这里已经被高温侵袭啦!又快到吹着空调吃西瓜的日子了呢。《Cell Stem Cell》杂志是2007年Cell出版社新增两名新成员之一(另外一个杂志是Cell Host & Microbe),这一杂志内容涵盖了从最基本的细胞和发育机制到医疗软件临床应用等整个干细胞生物学研究内容。这一杂志特别关注胚胎干细胞、组织特异性和癌症干细胞的最新成果。《Cell Stem Cell》自创刊以来就倍受关注,影响因子迅速提升,从0一冲至16.826,又达到了23.394。其中最受关注
4.23 丁香实验科研午间快讯(每日更新)①Cell Stem Cell八大热点文章(4月)《Cell Stem Cell》杂志是2007年Cell出版社新增两名新成员之一(另外一个杂志是Cell Host & Microbe),这一杂志内容涵盖了从最基本的细胞和发育机制到医疗软件临床应用等整个干细胞生物学研究内容。这一杂志特别关注胚胎干细胞、组织特异性和癌症干细胞的最新成果。《Cell Stem Cell》自创刊以来就倍受关注,影响因子迅速提升,从0一冲至16.826,又达到了23.394
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