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- ATCC Number：
HB-10894™
- 器官来源：
外周血
- 库存：
大量
- 年限：
adult
- 组织来源：
mononuclear cells
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 免疫类型：
IgG1 kappa
- 细胞形态：
淋巴样
- 生长状态：
悬浮生长
| Designations: | 558-D 100, 10, 1 [558-D] | ||
| Depositors: | New York University | ||
| Isotype: | IgG1 kappa | ||
| Biosafety Level: | 2 [Cells contain Human Immunodeficiency virus type 1 (HIV-1) ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | human (B cell); human x mouse (heterohybridoma) | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Tissue: mononuclear cells Cell Type: hybridoma: B lymphocyte; Epstein-Barr virus (EBV) transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | adult | ||
| Comments: | Human peripheral blood mononuclear cells from HIV seropositive patients were transformed with Epstein-Barr virus (EBV). [56270 ] Antibody producing clones were expanded and fused with SHM-D33 heterohybridoma cells (ATCC CRL-1668). [56270 ] The antibody is specific for the CD4-binding domain of the gp120 protein of HIV-1; it inhibits CD4-rgp120 binding. It has broad HIV group specificity and reacts with the HTLV-IIIB, MN and SF-2 strains of HIV-1. [56270 ] The antibody can be used in ELISA and neutralization assays. [56270 ] |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 2 X 10 exp6 viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 56270: Zolla-Pazner S, et al. Human monoclonal antibodies to the CD4-binding domain of HIV, uses thereof and synergistic neutralization of HIV. US Patent 6,241,986 dated Jun 5 2001 61449: Wang H, et al. Human monoclonal and polyclonal anti-human immunodeficiency virus-1 antibodies share a common clonotypic specificity. Eur. J. Immunol. 22: 1749-1755, 1992. PubMed: 1378015 |
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