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- 相关疾病：
多发性骨髓瘤
- ATCC Number：
TIB-6™
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- 品系：
BALB/c
- 库存：
大量
- 免疫类型：
IgA; lambda light chain
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
| Designations: | J558 | ||
| Depositors: | M Cohn | ||
| Isotype: | IgA; lambda light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Disease: plasmacytoma; myeloma Strain: BALB/c Cell Type: B lymphocyte; |
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| Cellular Products: | immunoglobulin; monoclonal antibody; against alpha-1,3-dextran | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2d; PC.1 | ||
| Cytogenetic Analysis: | diploid | ||
| Comments: | The plasmacytoma was induced with mineral oil in a BALB/c mouse. The cells are sensitive to dexamethasone and cortisol. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2040 |
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| References: | 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956 22228: Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326 22522: Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 22586: Carson D, Weigert M. Immunochemical analysis of the cross-reacting idiotypes of mouse myeloma proteins with anti-dextran activity and normal anti-dextran antibody. Proc. Natl. Acad. Sci. USA 70: 235-239, 1973. PubMed: 4119224 22744: Lundblad A, et al. Immunochemical studies on mouse myeloma proteins with specificity for dextran or for levan. Immunochemistry 9: 535-544, 1972. PubMed: 5036150 23254: Cesari IM, Weigert M. Mouse lambda-chain sequences. Proc. Natl. Acad. Sci. USA 70: 2112-2116, 1973. PubMed: 4516208 23494: Weigert MG, et al. Variability in the lambda light chain sequences of mouse antibody. Nature 228: 1045-1047, 1970. PubMed: 5483159 26330: . . Science 177: 1972.. |
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J558
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