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- 技术资料
- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- ATCC Number：
CRL-8621™
- 年限：
fetus, first trimester
- 器官来源：
大脑
- 库存：
大量
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞类型：
其他细胞类型
| Designations: | SVG p12 | ||
| Depositors: | The United States of America | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: brain Cell Type: astroglia; SV40 transformed |
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| Cellular Products: | SV40 T protein; glial fibrillary acidic protein (GFAP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | fetus, first trimester | ||
| Comments: | The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40. The cells express SV40 T antigen. The are susceptible to infection by JC virus, and may be useful in, detecting and cultivating other human neurotropic viruses. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nirogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 21915: Major EO. Immortal line of human fetal glial cells. US Patent 4,707,448 dated Nov 17 1987 | ||
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