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- 询价记录
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 运输方式：
冻存运输
- 品系：
AxC
- 器官来源：
肝
- 相关疾病：
肝癌
- 库存：
大量
- ATCC Number：
CRL-1600™
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
| Designations: | H4-II-E-C3 | ||
| Depositors: | JE Becker | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Strain: AxC Disease: hepatoma |
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| Cellular Products: | tyrosine aminotransferase; albumin; transferrin; prothrombin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Gender: | male | ||
| Comments: | Tyrosine amino transferase is inducible with glucorticoids, insulin or cAMP derivatives. The cells are productively infected with a retrovirus. |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040 |
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| References: | 1084: Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488 1217: Reuber MD. A transplantable bile-secreting hepatocellular carcinoma in the rat. J. Natl. Cancer Inst. 26: 891-899, 1961. PubMed: 13740982 26102: Weinstein IB, et al. Type C virus from cell cultures of chemically induced rat hepatomas. Science 178: 1098-1100, 1972. PubMed: 4343844 33030: Klemm DJ, et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 58076: Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233 |
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文献和实验E.Z.N.A. Plant RNA Midi Protocol II (for difficult samples)
ml collection tube (Not supplied), and add 3.5 ml RNA Wash Buffer II diluted with ethanol. Centrifuge at 4,000 x g for 5 minutes at room temperature and discard flow-through. Reuse the collection tube in step 11. 11. Wash column with a second 3 ml of RNA
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
yields. 3. Transfer the sample to a 1.5 ml centrifuge tube. Add 500 ul Solution II and mix gently but throughly by inverting and rotating the tube 7-10 times to obtain a cleared lysate. This may require a 2-3 min incubation at room temperature
Methods for Assessing Autophagy and Autophagic Cell Death Cytosolic LC3 Ratio as a Sensitive Index of Macroautophagy in Isolated Rat Hepatocytes and H4-II-E Cells 本文经作者系 @丁香园中级站友 biofish。
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