- 询价
- 2026年06月27日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 细胞形态：
淋巴样
- ATCC Number：
CRL-2016™
- 库存：
大量
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
| Designations: | Sp2/mIL-6 | ||
| Depositors: | JF Harris | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | mouse interleukin 6 (interleukin-6, IL-6) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Note: These cells are distributed for research purposes only. Dr. Harris releases the line subject to the following: 1.) The Sp2/mIl-6 cells or any cells derived from SP2/mIL-6 or any antibody derived from it must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. 2.) Any proposed commercial use of these cells should first be negotiated with the London Regional Cancer Centre, 790 Commissioners Road, East, London, Ontario. N6A 4L6 Canada. 3.) In all papers reporting any use of these or derived clones a direct reference will be made to the original publication (J. Immunol. Methods 148: 199-207, 1992). | ||
| Isolation: | Toronto Ontario, Canada | ||
| Antigen Expression: | H-2, d haplotype; Mus musculus, expressed | ||
| Comments: | The Sp2/mIL-6 line was established by R. Hawley and T. Hawley of the Sunnybrook Health Centre, Toronto, Canada. It is a mouse fusion cell line derived from Sp2/0 cells that had been transfected with high-titer L6PNL recombinant retrovirus carrying a mouse interleukin-6 (IL-6) cDNA under the transcriptional control of the viral LTR and the neo gene encoding bacterial neomycin phosphotransferase (G418 resistance). Sp2/mIL-6 cells have stably incorporated in their genome approximately six copies of the retroviral vector L6PNL and constitutively secrete large quantities of mouse IL-6 (10,000 units/ml). Sp2/IL-6 cells secrete IL-6 and are an improved myeloma fusion parent in terms of both the relative number of viable B cell hybridomas producing immunoglobulin and the proportion of hybridomas producing antigen-specific monoclonal antibodies. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 2 X 10(5) cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density) |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 23169: Harris JF, et al. Increased frequency of both total and specific monoclonal antibody producing hybridomas using a fusion partner that constitutively expresses recombinant IL-6. J. Immunol. Methods 148: 199-207, 1992. PubMed: 1373425 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验文献： [1]Xu H, Li YF, Yi XY, Zheng XN, Yang Y, Wang Y, Liao DZ, Zhang JP, Tan P, Xiong XY, Jin X, Gong LN, Qiu S, Cao DH, Li H, Wei Q, Yang L, Ai JZ. ADP-dependent glucokinase controls metabolic fitness in prostate cancer progression. Mil Med Res. 2023 Dec 12
通用名称译音的，使用方便，缺点是名称和结构之间没有任何联系。 杂环化合物的环上原子编号，除个别稠杂环如异喹啉外，一般从杂原子开始。 环上只有一个杂原子时，杂原子的编号为1。有时也以希腊字母α、β及γ编号，邻近杂原子的碳原子为α位，其次为β位，再次为γ位。 当杂环上连有-R，-X，-OH，-NH 2 等取代基时，以杂环为母体，标明取代基位次；如果连有-CHO，-COOH，-SO 3 H等时，则把杂环作为取代基。环
Bioassays Quick Guide Cytokine Indicator Cells Cell Density (x105 ) Feeder Cytokine Assay Medium FBS (%) Incubation Time (hours) Spec U/mg (10e) Recomb. Cytokine
技术资料暂无技术资料 索取技术资料
