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- 2026年02月20日
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- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- ATCC Number：
HB-9725™
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 库存：
大量
- 生长状态：
悬浮生长
| Designations: | 31-90-25 | ||
| Depositors: | Abbott Labs. | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human immunodeficiency virus 1 (HIV 1, HIV-1) p24 protein | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Spleen cells were fused with Sp2/0 myeloma cells. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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| References: | 3999: Stewart JL, et al. Immunoassay for HIV-1 antigens using F(AB').sub.2 fragments as probe. US Patent 4,983,529 dated Jan 8 1991 | ||
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文献和实验3.82 3.47 3.25 3.09 2.97 2.87 2.80 2.73 2.64 2.53 2.42 2.31 2.18 2.04 21 22 4.38 3.73 3.44 3.21 3.05 2.93 2.84 2.76 2.70 2.60 2.50 2.39 2.27 2.14 2.00 22 23 4.35 3.75 3.41 3.18 3.02 2.90 2.81 2.73 2.67 2.57 2.47 2.36 2.24 2.11 1.97 23 24
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41 32 27 22 14 37 23 24 19 12 31 22 19 15 9 27 19 15 12 6 0.80 0.85 37 29 24 20 13 33 25 24 17 11 28 21 17 13 8 24 17 14 11 6 0.85 0.90 34 26 22 18 12 29 23 19 16 10 25 19 16 12 7 21 15 13 10 5 0.90 0.95 31 24 20 17 11 27 21 18 14 9 23 17 14 11
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