产品封面图

SNL76/7

收藏
  • 询价
  • 2026年03月01日
    avatar
    ATCC细胞库
    15 年金牌会员

  • 企业认证

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 物种来源：

      小鼠

    • 是否是肿瘤细胞：

      0

    • 细胞形态：

      成纤维样

    • ATCC Number：

      SCRC-1049™

    • 品系：

      SIM (Sandos Inbred Mice)

    • 细胞类型：

      成纤维细胞

    • 年限：

      embryo

    • 生长状态：

      贴壁生长

    • 运输方式：

      冻存运输

    • 库存：

      大量

    • 器官来源：

      胚胎

    Designations: SNL76/7
    Depositors:  A Bradley
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Mus musculus
    Morphology: fibroblast
    Source: Organ: embryo
    Strain SIM (Sandos Inbred Mice)
    Cell Type: fibroblast
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Restrictions: Prior to purchase, for-profit commercial institutions must obtain a license agreement. For instructions on how to proceed, please contact Baylor Licensing Group, blg@bcm.tmc.edu
    Isolation: Isolation date: 1988
    Applications: Note: ATCC tested that this cell line is resistant to G 418 (neomycin): 350 �g/ml.
    SNL76/7 was clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF) at an abundant level.
    This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells and induced pluripotent stem (iPS) cells.
    Age: embryo
    Gender: male and female mixed
    Comments: SNL76/7 was clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF) at an abundant level.
    The STO cell line was transfected with a G418-resistance cassette, RV4.0 and a LIF expression construct.
    This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells and induced pluripotent stem (iPS) cells.
    Inclusion of G418 in the medium is not necessary for normal cell growth.
    Note: ATCC tested that this cell line is resistant to G 418 (neomycin): 350 �g/ml.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Subculturing: Establishing culture from frozen cells: To insure the highest level of viability, be sure to warm media to 37�C before using it on the cells. Flasks do not need to be coated before plating MEFs.
    • Thaw the vial by gentle agitation in a 37�C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water.
    • Remove the vial from the water bath as soon as the contents are half way thawed (approximately 90 seconds), and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
    • Transfer the vial's contents plus 5 ml of complete medium (see ATCC complete growth medium for recipe) to a 15 ml centrifuge tube. Use an additional 1 ml of medium to rinse the vial and transfer the liquid to the 15 ml tube. Add 4 ml of complete medium to bring the total volume to 10 ml.
    • Gently mix and pellet the cells by centrifugation @ 270x g for 5 minutes.
    • Discard the supernatant and resuspend the cells with 10ml fresh growth medium (warm) and plate cells at seed density of 0.8 X 104 cells/cm2 .
    • Add 5 ml more fresh growth medium (warm) to flask.
    • Incubate 37�C in a 5% CO2 in air atmosphere.
    • Fluid change twice a week or when pH decreases. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes. to allow the medium to reach its normal pH (7.0 to 7.6).

    Subculturing Procedure:
    Cells should be split before they reach confluency.
    To insure the highest level of viability, warm culture medium, PBS and Trypsin-EDTA to 37.0°C before use.
    Volumes used in this protocol are for 75 sq. cm. flasks; proportionally reduce or increase reagent volumes for culture flasks of other sizes.
    • Remove and discard culture medium.
    • Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
    • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
    • Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting.
    • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of approximately 6 X10(3) cells/sq. cm.is recommended.
    • Incubate cultures at 37.0°C in a 5% CO2 in air atmosphere.

    Subcultivation Ratio: 1:10
    Interval: every 3 days
    Preservation: Freeze medium: complete growth medium supplemented with an additional 35% fetal bovine serum and 10% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Derivative: ATCC SCRC-1050
    Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
    Recommended serum: ATCC 30-2020
    0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
    Cell culture tested DMSO: ATCC 4-X
    Phosphate-buffered saline: ATCC 30-2200
    References: 22928: Williams RL, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687, 1988. PubMed: 3143916
    16173313: McMahon AP, Bradley A. The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell. 62(6): 1073-1085, 1990. PubMed: 2205396
    16173314: Takahashi K, et al. Induction of pluripotent stem cells from fibroblast cultures. Nat. Protoc. 2(12): 3081-3089, 2007. PubMed: 18079707
    16173315: Thomas KR, Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51(3): 503-512, 1987. PubMed: 2822260
    16173317: Okita K, et al, Generation of germline-competent induced pluripotent stem cells. Nature 448(7151): 313-317, 2007. PubMed: 17554338

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    相关实验

    • Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood

      of exogenous genes. Infected cells are cultured in hematopoietic medium (X-VIVO 10 containing 50 ng/mL IL-6, 50 ng/mL soluble IL-6 R, 50 ng/mL SCF, 10 ng/mL TPO, and 20 ng/mL Flt-3-ligand) for 5 days after viral infection. Then, cells are transferred onto SNL76

    • FEEDER  PREPARATION  BY  GAMMA  IRRADIATION

      FEEDER  PREPARATION  BY  GAMMA  IRRADIATION All procedures should be carried out using sterile techniques at all times.  Media for STOs/ SNL 76/7 cells is DMEM (high glucose, no pyruvate), 7% FBS and 1X GPS. 1. Gelatinize plates

    • ES Cell Culture and Manipulation

      dense, split cells no more than 1:10) Freezing ES cells Freeze as for STO/SNL cells, ES freezing media is regular ES media with 20% FBS and 10% DMSO. It is very important that the cells be cooled slowly, to prevent the formation of ice

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    SNL 76/7小鼠胚胎成纤维细胞（ATCC引进，提供STR）
    ￥5200
    武汉赛奥斯生物科技有限公司
    2026年01月30日询价
    Or De
    询价
    ATCC细胞库
    2025年11月03日询价
    ATCC原装细胞，ATCC代理，ATCC中国,ATCC菌株厂家,ATCC厂家，ATCC代理厂家，细胞ATCC感受,ATCC
    询价
    吉奥蓝图（广东）生命科学技术中心
    2025年08月28日询价
    ATCC
    询价
    北京中源合聚生物科技有限公司
    2026年01月22日询价
    HCCLM3细胞系、HCCLM3细胞株、HCCLM3细胞
    ￥1400
    上海盖宁生物科技有限公司
    2026年01月27日询价
    SNL76/7
    询价