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- 2025年10月04日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 器官来源：
肝
- 运输方式：
冻存运输
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1603™
- 库存：
大量
- 品系：
Sprague-Dawley
- 生长状态：
悬浮生长
| Designations: | N1-S1 Fudr | ||
| Depositors: | JE Becker | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | |
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| Source: | Organ: liver Strain: Sprague-Dawley Disease: hepatoma; Novikoff hepatoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | male | ||
| Comments: | The cells are resistant to 5-fluoro-2'-deoxyuridine (FUdR) at 0.05 mM and to 5-fluorouracil. This line is derivative of N1-S1 (ATCC CRL-1604 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
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| Subculturing: | Medium Renewal: Twice per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp4 viable cells/ml and subculture at 1 X 10 exp6 cells/ml (about 3 days). Cells can be grown in Florence flasks on a rotary shaker at 180 to 190 RPM. |
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| References: | 1084: Potter VR, Morse PA Jr.. Pyrimidine metabolism in tissue culture cells derived from rat hepatomas. I. Suspension cell cultures derived from the Novikoff hepatoma. Cancer Res. 25: 499-508, 1965. PubMed: 14297488 26107: Cory JG, et al. Evidence for role of purine nucleoside phosphorylase in sensitivity of Novikoff hepatoma cells to 5-fluorouracil. Adv. Enzyme Regul. 15: 153-166, 1976. PubMed: 197803 |
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