- 询价
- 2025年10月04日
企业认证
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
褐鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 库存：
大量
- ATCC Number：
CRL-1604™
- 品系：
: Sprague-Dawley
- 生长状态：
悬浮生长
| Designations: | N1-S1 | ||
| Depositors: | JE Becker | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Rattus norvegicus | ||
| Morphology: | |
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| Source: | Strain : Sprague-Dawley | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Gender: | male | ||
| Comments: | The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 (4) viable cells/ml and subculture at 1 to 1.5 X 10 (6) cells/ml ( after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them. | ||
| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% | ||
| Related Products: | ATCC CRL-1603, N1-S1 | ||
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文献和实验sadows 看一篇文献摘要里多次提到PC12 N1,N1代表什么,是PC12细胞中的一型么?那一型呢?PC12不是只有分化的和没分化的么?望达人赐教.谢谢摘要如下: JNKs, also known as SAPKs, are activated in response to a wide variety of factors including growth factors, cytokines, UV radiation, and heat
S1 Nuclease Protection Mapping
The use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp (1 ), it has undergone many refinements over the years. S1 nuclease mapping requires a relatively
digested plasmid (cut 5 µg of plasmid in volume of 20 µl) 51 µl labeled oligo 10 µl 10X Taq Buffer 6 µl 25 mM MgCl2 10 µl DMSO 2 µl 10 mM dNTP's 1 µl Taq polymerase 14 cycles of PCR Run on 6-8% polyacrylamide/urea gel Cut out probe and elute S1
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