- 询价
- 2026年06月24日
企业认证
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 相关疾病：
其他疾病
- 运输方式：
冻存运输
- ATCC Number：
CRL-2768™
- 库存：
大量
- 细胞形态：
神经元
- 生长状态：
贴壁生长
- 器官来源：
其他
- 物种来源：
褐鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
| Designations: | RT4-D6P2T | ||
| Depositors: | PI Patel | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus | ||
| Morphology: | neuronal |
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| Source: | Organ: peripheral nervous system Disease: Schwannoma Cell Type: neuronal Schwann cell; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Comments: | Products: cyclic nucleotide phosphodiesterase 1 (Cnp1) (CNPase); positive [PubMed: 2824698]Laminin; positive [PubMed: 2824698]Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843] Myelin basic protein (Mbp); protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); protein and mRNA, positive [PubMed: 12210843 peripheral myelin protein 22 (Pmp22) mRNA, positive; protein, negative [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b); protein, positive [PubMed: 12210843]Receptors expressed: Nerve growth factor receptor (Ngfr) ; protein, negative; mRNA, positive [PubMed: 12210843]Comments: RT4-D6P2T is an schwannoma cell line derived from a N-ethyl-N-nitrosourea (ENU) induced rat peripheral neurotumor, RT4. The tumor was grown in culture and a cell line RT4-AC was isolated that subsequently developed in a distinct cell type, RT4-D that produces glial specific S100. RT4-D was subcloned to produce RT4-D6 that was subcloned to produce RT-4-D6P2 and then finally subcloned to produce the D6P2T subclone [PubMed: 2824698]. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 64424: Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843 71903: Bansal R, Pfeiffer SE. Regulated galactolipid synthesis and cell surface expression in Schwann cell line D6P2T. J. Neurochem. 49: 1902-1911, 1987. PubMed: 2824698 |
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文献和实验X-1(-2,-3)pGEX-5pGEX-3pGEX-6P-1(-2,-3)pGEX-5pGEX-3pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)pGL3-Basic(MCSI)RVP3(PGL3+)?GLP2(PGL3-)?pGL3-Basic(MCSII)RVP4,PLXSN-3'?pGL4 pGlow(-TOPO)T7pGlow-3pGM-TT7SP6pGWCSP6T7pHook-2T7/CMV-Profornot availablepIND/V5-His_A(_B,_C
Inverse PCR for PAC-end sequencing
GTG PCYPAC2SP6R GAT CGA AAC GGC AGA TCG PCYPAC2T7L CCT TGA GAG CCT TCA ACC PCYOAC2T7R CGA GCT TGA CAT TGT AGG AC Stages in protocol (1) Restriction digest of PAC DNA (2) ligation of restriction digests (3) PCR
?PurificationSystems?Strep?TactinResinsandPurificationKits 四.PGEX系列表达载体 TEcoR?pGEX-1I/BAP pGEX-2T pGEX-2TK pGEX-3X pGEX-4T-1 pGEX-4T-2 pGEX-4T-3 pGEX-5X-1 pGEX-5X-2 pGEX-5
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