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- 详细信息
- 文献和实验
- 技术资料
- 免疫类型:
IgG1
- 细胞形态:
淋巴样
- 库存:
大量
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 运输方式:
冻存运输
- 生长状态:
悬浮生长
- ATCC Number:
HB-8523™
| Designations: | RLTM02 [Anti-PAP 2, DB91 5A3.3.2] | ||
| Depositors: | Cetus Corp. | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human prostatic acid phosphatase (PAP) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with purified PAP from human semen. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X |
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| References: | 3896: M'Timkulu T, et al. Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay. US Patent 4,690,890 dated Sep 1 1987 | ||
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文献和实验[摘要] 目的:为了解受血者输血前血液感染情况,确保输血安全,探讨其检测的必要性。方法:用紫外乳酸脱氢酶法测ALT;用ELISA法测HBsAg、Anti-HCV、Anti-TP、Anti-HIV。结果:在222例受血者输血前血清中检测出HBsAg阳性率为13.91%、Anti-HcV阳性率为1.35%、Anti-TP阳性率为2.70%、Anti-HIV未检出阳性。结论:检测受血者输血前血清感染性标志物,可了解受血者输血前状况,保障临床输血安全,减少不必要的医疗纠纷发生和防止
化酶电极法、总蛋白( TP) 双缩脲法、清蛋白(Alb) 溴甲酚紫法、镁(Mg) Calmagite 比色法,试剂及校准品由贝克曼公司提供;总胆红素( TB) 矾酸盐氧化法、直接胆红素(DB) 矾酸盐氧化法、丙氨酸氨基转移酶(AL T) 速率法、天门冬氨酸氨基转移酶(AST) 速率法、碱性磷酸酶(AL P) 速率法、γ-谷氨酰转肽酶(γ-GT) 速率法、淀粉酶(AMY) 法、胆固醇(CHO) 氧化酶法、三酰甘油( TG) CPO-PAP 法、肌酸激酶(CK) 速率法、乳酸脱氢酶(LDH) 速率法、尿酸
Fluorescent In Situ Transcription in Cells and Tissues
cellular mRNA transcripts in tissue sections and cell cultures using unique short anti?sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA
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