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- 技术资料
- 库存：
大量
- 运输方式：
冻存运输
- 相关疾病：
腺癌
- 器官来源：
肺
- ATCC Number：
HTB-57™
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 年限：
60 years
- 物种来源：
人
- 是否是肿瘤细胞：
0
| Designations: | SK-LU-1 | ||
| Depositors: | G Trempe, LJ Old | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Disease: adenocarcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. | ||
| Tumorigenic: | Yes | ||
| Antigen Expression: | Blood Type O; Rh+; HLA Aw24, Aw32, B27, Bw41 | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10 D13S317: 10 D16S539: 8 D5S818: 11 D7S820: 9 THO1: 7 TPOX: 8,10 vWA: 16,17 |
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| Cytogenetic Analysis: | The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation. | ||
| Isoenzymes: | AK-1, 1 ES-D, 2 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 2 PGM3, 1 |
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| Age: | 60 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | Mycoplasma contamination was detected and eliminated in 1971. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: Twice per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 |
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SK-LU-1
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