- 询价
- 2026年03月01日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
CRL-10354™
- 年限：
5 days
- 品系：
Sprague-Dawley
- 运输方式：
冻存运输
- 器官来源：
肺
- 库存：
大量
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
| Designations: | RL-65 | ||
| Depositors: | Genentech, Inc. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | epithelial |
||
| Source: | Organ: lung Strain: Sprague-Dawley |
||
| Cellular Products: | cytoskeletal proteins (alpha keratin, actin, desmin, vimentin, tubulin); fibronectin; laminin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | No | ||
| Age: | 5 days | ||
| Comments: | The cells exhibit different characteristic when grown with or without retinoic acid. In the presence of retinoic acid (50 nM), the cells resemble low non-keratinized or squamous epithelium with densely packed colonies. In the absence of retinoic acid, the cells form a keratinized epithelium. Long term cultures in the absence of retinoic acid form a stratified highly keratinized epithelium with large networks of epithelial filaments. The cells are maintained in a serum free medium. If grown in media containing serum, the properties of the cells will change. |
||
| Propagation: | ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 1.2 g/L sodium bicarbonate and supplemented with 0.005 mg/ml insulin, 0.01 mg/ml human transferrin, 0.1 mM ethanolamine, 0.1 mM phosphoethanolamine, 25 nM selenium, 500 nM hydrocortisone, 0.005 mM forskolin, and bovine pituitary extract (0.15 mg protein per ml). Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture to sit at room temperature (or 37C) until the cells detach (about 5 minutes). Centrifuge, resuspend cells in fresh medium and dispense into new flasks. |
||
| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 | ||
| References: | 22065: Mather JP, Roberts PE. Method of isolating lung cell line. US Patent 5,364,785 dated Nov 15 1994 22473: Roberts PE, et al. A novel epithelial cell from neonatal rat lung: isolation and differentiated phenotype. Am. J. Physiol. 259: L415-L425, 1990. PubMed: 2260675 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验foreye 我想免疫组化法检测NFkBp65，请问santa cruz的这类抗体好用么？sc109和sc372分别识别的是NFkb p65的N末端和C末端，这两个有区别么？是否都是激活后暴露出来的？（记得C端是结合DNA的位置）请战友前辈指教！谢谢谢谢！ lishufang821 我最近也在做免疫组化法检测NFkBp65，买的中山的，现在正在摸浓度，赫赫 多交流阿。QQ：493093683
RNA Markers的使用方法(适用于RNA Marker RL1,000、RL6,000、RL10,000）
RNA Marker ( RL1,000; RL6,000; RL10,000)可以进行一般琼脂糖凝胶电泳和变性琼脂糖凝胶电泳。以RL10,000为例，具体使用方法如下：普通琼脂糖凝胶电泳时1.按下列组份配置RNA Marker样品。2.均匀混合后65℃加热10分钟，迅速冷却至室温（最好用PCR仪)。3.使用高质量的琼脂糖，用1×TAE Buffer制备3%凝胶*，制胶时凝胶中请加入溴乙锭(最终浓度：1 μg/ml)。4.将上述操作2配制的Marker样品加样后，在1×TAE Buffer中
CD65/CD65s 分子 CD65 常用单克隆抗体或代号： VIM2,HE10,CF4 主要表达细胞： PMN [M] 分子质量(kDa)和结构： 岩藻糖基神经节苷脂 功 能： 中性粒细胞活化 CD65s 常用单克隆抗体或代号： 88H7 主要表达细胞： PMN,M [M] 分子质量(kDa)和结构： Siapoly- sialyl- n
技术资料暂无技术资料 索取技术资料
