- 询价
- 2026年06月19日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 细胞形态：
上皮样
- 相关疾病：
肿瘤
- ATCC Number：
CRL-2579™
- 器官来源：
结肠
- 库存：
大量
| Designations: | RKO-AS45-1 | ||
| Depositors: | MC Hollander, AJ Fornace | ||
| Biosafety Level: | 2 [Contains CMV viral sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: colon Disease: carcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Oncogene: | p53 + (underexpressed) | ||
| Comments: | The RKO-AS45-1 cell line was generated from the colon carcinoma RKO (ATCC CRL-2577 ) cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV. [51464 ] The cells contain a stably integrated cytomegalovirus (CMV) promotor driven expression vector. The cells overexpress Gadd45 RNA and protein. The GADD45 gene is p53-regulated and the Gadd45 protein interacts with proteins involved in both cell-cycle regulation and DNA repair. The RKO-AS45-1 cell line has reduced ability to repair U.V.-induced DNA damage. [51464 ] The RKO-AS45-1 cell line can be used together with its parental cell line, RKO (ATCC CRL-2577 ) to investigate the effects of Gadd45 on DNA repair, apoptosis and drug sensitivity. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 parental cell line:ATCC CRL-2577 |
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| References: | 51464: Smith ML, et al. Antisense GADD45 expression results in decreased DNA repair and sensitizes cells to u.v.-irradiation or cisplatin. Oncogene 13: 2255-2263, 1996. PubMed: 8950993 53491: Smith ML, et al. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen. Science 266: 1376-1380, 1994. PubMed: 7973727 53492: Bae I, et al. An abnormality in the p53 pathway following gamma-irradiation in many wild-type p53 human melanoma lines. Cancer Res. 56: 840-847, 1996. PubMed: 8631022 |
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文献和实验CRL-2578 Homo sapiens (human) RKO-E6 CRL-2579 Homo sapiens (human) RKO-AS45-1 CRL-2780 Homo sapiens (human) ATRFLOX [Mutatect] CRL-5972 Homo sapiens (human) SNU-C1 CRL-7213 Homo sapiens (human) Hs 255.T CRL-7214 Homo sapiens (human
correct reconstruction of A53T and A30P.（8）结果:DNA测序结果证明构建载体插入正确α-突触核蛋白突变体A53T与A30P基因。（9）METHODS: RKO cells were treated with selective DNA methyltransferase (DNMTs) inhibitor, 5-Aza-2′-deoxycytidine (5-Aza-CdR), for 72 h. Methylation-specific PCR (MSP), T
疟、间日疟和三日疟。后二者又称良性疟。 疟原虫的生活史可分为雌按蚊体内的有性生殖阶段和人体内的无性生殖阶段（见图45-1）。 图45－1 疟原虫生活史和各类抗疟药的作用部位 1．人体内的无性生殖阶段 可分为以下各期： （1）原发性红细胞外期：受感染按蚊叮咬人时，将其唾液中的子孢子输入人体。要30分钟内子孢子即侵入肝细胞中开始其红细胞前期发育和裂体增殖。经过10～14天，生成大量裂殖子。此期不发生症状，为疟疾的潜伏期。对此期有杀灭作用的药物
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