- 询价
- 2025年10月04日
14 年金牌会员
企业认证
- 详细信息
- 询价记录
- 技术资料
- 组织来源：
choroid; retina
- 物种来源：
猕猴
- 是否是肿瘤细胞：
0
- 细胞类型：
内皮细胞
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 相关疾病：
正常
- ATCC Number：
CRL-1780™
- 库存：
大量
- 年限：
fetus
| Designations: | RF/6A | ||
| Depositors: | F Hu | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Macaca mulatta | ||
| Morphology: | epithelial |
||
| Source: | Tissue: choroid; retina Disease: normal Cell Type: endothelial |
||
| Cellular Products: | factor VIII; fibronectin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes. | ||
| Age: | fetus | ||
| Comments: | This line spontaneously transformed at an early age and has been passaged over 540 times. Weibel-Palade granules are seen by electron microscopy. Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
||
| Subculturing: | Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice weekly |
||
| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
||
| References: | 22315: Lou DA, Hu FN. Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
RF/6A
询价
