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- 2026年03月01日
企业认证
- 详细信息
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- 技术资料
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- 年限：
6 years
- 器官来源：
皮肤
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 相关疾病：
马凡氏综合征
- 库存：
大量
- ATCC Number：
CRL-1289™
| Designations: | Ra Lot | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: skin Disease: Marfan syndrome |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 6 years | ||
| Gender: | male | ||
| Comments: | The tissue donor suffered from a severe form of Marfan syndrome. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Remove spent medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. |
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白介素-1ra (IL-1ra)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 IL-1ra 单抗包被于酶标板上，标准品和样品中的 IL-1ra与单抗结合，加入生物
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠白介素 -1ra(IL-1ra)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 IL-1ra 单抗包被于酶标板上，标准品和样品中的 IL-1ra 与单抗结合，加入生物素化的抗大鼠 IL-1ra ，形成免疫
Identification and Cloning of RA-Regulated Genes by mRNA-Differential Display
), reverse transcrrptase-polymerase chain reaction (RT-PCR) analysis ( 3 ), and RNase protection ( 4 ) have been utilized to study specific differences in gene expression following RA treatment. Although these techniques have proven useful
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