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- 详细信息
- 文献和实验
- 技术资料
- 相关疾病:
正常
- 器官来源:
肺
- ATCC Number:
CCL-193™
- 生长状态:
贴壁生长
- 年限:
fetus
- 库存:
大量
- 物种来源:
其他
- 是否是肿瘤细胞:
0
- 细胞类型:
成纤维细胞
- 细胞形态:
成纤维样
- 运输方式:
冻存运输
| Designations: | R9ab | ||
| Depositors: | EM Earley | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Oryctolagus cuniculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: lung Disease: normal Cell Type: fibroblast |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | normal; diploid; stable | ||
| Age: | fetus | ||
| Gender: | female | ||
| Comments: | R9ab was derived from explants of biopsy material. Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line. The donor indicated that the life expectancy of this line is at least 60 passages. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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| References: | 32878: Castagliuolo I, et al. Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum. Infect. Immun. 64: 5225-5232, 1996. PubMed: 8945570 | ||
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