万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 运输方式:
冻存运输
- 细胞形态:
成纤维样
- ATCC Number:
CRL-1657™
- 生长状态:
贴壁生长
- 器官来源:
肺
- 物种来源:
仓鼠
- 是否是肿瘤细胞:
0
- 库存:
大量
| Designations: | R 1610 | ||
| Depositors: | M Rosenberg | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | fibroblast |
||
| Source: | Organ: lung | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Gender: | male | ||
| Comments: | This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93 ) that had been treated with ethylmethanesulfonate. The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine. This is a Chinese hamster somatic cell line. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
||
| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liqid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-93 |
||
| References: | 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760 25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916 |
||
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验指在 DNA双链之一部分,由与其互补的 RNA与之结合而产生的核酸分子的环形结构。为与由 DNA而成的 D环相对应的名称。因 RNA、 DNA杂合的双链比 DNA双链稳定,所以在比 DNA的双链开始分开时稍高的温度下, RNA分子仍与 DNA的互补部分形成稳定的双链。即如图 A所示, RNA与具同一性质的 DNA部分置换,形成 R环。在基因 DNA具有外显子 -内含子( Exons- introns)结构时,则如图 B所示只在内含子部分的 DNA以双链环残存着,产生与 Excons数相应
R因子 R factor 抗药因子或抗多药剂因子的简称。 R是 resista- nce一词的首字。它是一种细胞质性的质粒,这种质粒具有使寄主菌对链霉素、氯霉素、四环素等抗菌素或磺胺剂产生抗药性的基因群,和 F因子一样,通过接台进行转移,获得该因子的细菌同时获得对多种药剂的抗性,因而给治疗带来很大麻烦。 R因子的实体为环状双链 DNA分子。自从 50年代后半期在日本发现 R因子以来,渡道力等( 1969)曾对此进行了研究。其它国家也分离了许多 R因子。现在已知有各式
r选择 r-selection 由 R.H.MacArthur和 E.O.wilson( 1967)创造的术语. r选择与 K选择是对立的.内部的自然增加率 r有利于自然的增大只限于种群密度远远低于环境容纳能力的时候,在种群维持接近于环境容纳能力的高密度水平时.而高效率地利用有限的资源毋宁说选择会有利于少数后代确保其存活的基因型。基于这一认识,根据 Logistic曲线.前者特命名为 r选择,后者命名为 K选择. r选择是指对在一个新的栖息场所(如岛屿)定居过程中,或对不断变动其不安
技术资料暂无技术资料 索取技术资料




