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- 细胞形态：
淋巴样
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
B淋巴细胞
- 器官来源：
外周血
- 年限：
adult
- 生长状态：
悬浮生长
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2631™
- 库存：
大量
- 运输方式：
冻存运输
| Designations: | Toledo | ||
| Depositors: | H Ben-Bassat | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | lymphoblast |
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| Source: | Organ: peripheral blood Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma Cell Type: B lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1990 | ||
| Antigen Expression: | CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289 ] | ||
| DNA Profile (STR): | D5S818:10,12 D7S820: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 Amelogenin: X CSF1PO: 12 D13S317:11 D16S539:9 |
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| Age: | adult | ||
| Gender: | female | ||
| Ethnicity: | White | ||
| Comments: | Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL). [53289 ] Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain. [53289 ] Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations. [53289 ] The cells do not express surface or cytoplasmic immunoglobulin. [53289 ] The cells are negative for Epstein-Barr virus. [53289 ] This cell line is a model system for studying non-Hodgkin lymphomas. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 24 to 30 hrs | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 | ||
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文献和实验METTLER TOLEDO AX205DR电子天平标准操作规程（SOP）
1、开机步骤：1.1打开稳压电源。1.2轻触“ON/OFF键”开启天平。1.3调节底座螺丝，使水平仪中的空气泡位于内圈中部。1.4按“↕”键打开玻璃防风罩，按“O/T”键进行去皮并回零。1.5将盛有待测物品的表面皿或滤纸放在称量盘上，按“↕”键关闭防风罩，待读数处左上方显示的稳定性探测图标消失即可读取称量结果。1.6称量完毕，应清扫天平内残留药品，关好天平侧门，清洁工作台。1.7长按OFF键关闭天平。1.8关闭电源，放下防尘窗。1.9 认真填写使用状态登记表，如实记录仪器状态。2、注意
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测定样品中的丽春红；L.Del Giovine 等采用醇氨混合液提取的方法处理样品，应用高效毛细管区带电泳测定冰激凌中的色素。本文建立了高效毛细管区带电泳分离测定冰激凌中丽春红色素的新方法，本法具有简便、快速、准确、消耗试剂少、测试费用低等优点，可为冰激凌类食品中色素的质量控制提供参考数据。 仪器及试剂 1. 仪器TH- 3000 高效毛细管电泳仪：紫外检测器，天惠分离科学研究所；电子天平：AG245，Mettler Toledo；架盘药物天平：0.1 g，北京医用天平厂
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