- 询价
- 2025年10月25日
14 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 库存：
大量
- 细胞形态：
上皮样
- 器官来源：
肝
- 运输方式：
冻存运输
- 相关疾病：
肝癌
- ATCC Number：
CRL-2117™
- 生长状态：
贴壁生长
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 品系：
Leghorn
- 细胞类型：
其他细胞类型
| Designations: | LMH | ||
| Depositors: | T Kitagawa, DL Williams | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Gallus gallus | ||
| Morphology: | epithelial |
||
| Source: | Organ: liver Strain: Leghorn Disease: hepatocellular carcinoma Cell Type: chemically induced |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Receptors: | estrogen receptor, positive | ||
| Tumorigenic: | Yes | ||
| Cytogenetic Analysis: | triploid; modal number = 116; six marker chromosomes | ||
| Comments: | LMH is a primary hepatocellular carcinoma epithelial cell line established in 1981 by Tomoyuki Kitagawa at the Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, Japan. Tumorous nodules were induced in the liver of a male leghorn chicken by long term treatment with diethylnitrosamine. The morphology of the cells is dendritic like. The cells express glucose-6-phosphatase and weak canalicular ATPase activity. The cells form tumors in athymic nude mice. LMH cells contain a low level of functional estrogen receptor and are inducible for the expression of the liver specific apolipoprotein II (apoII) gene. The cell line is useful for transfection studies. NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin. To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes and remove the liquid. Vessels prepared in this fashion may be used immediately. |
||
| Propagation: | ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10% |
||
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Flasks must be coated with 0.1% gelatin. |
||
| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22374: Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000 23063: Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775 23244: Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
LMH
询价
